Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates
BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial. RESULTS:Theeffects of reaction variables reaction time, tempe...
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Society of Chemical Industry
2013
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| Online Access: | http://eprints.um.edu.my/9632/1/00013161_103461.pdf http://eprints.um.edu.my/9632/ |
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| author | Gumel, A.M. Annuar, M.S.M. Heidelberg, T. |
| author_facet | Gumel, A.M. Annuar, M.S.M. Heidelberg, T. |
| author_sort | Gumel, A.M. |
| building | UM Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically
active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial.
RESULTS:Theeffects of reaction variables reaction time, temperature,enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1’-O-3-hydroxyacyl-sucrose) using Candida antarctica lipase B (EC 3.1.1.3)were studied. Using H2O2 as micro-initiator, enzyme-mediated synthesis yielded reaction rate, vapp of 0.076 x 10−5 mol L−1 s−1. The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non-functionalized material apart from
showing better compostability. Increasing the reaction temperature (>50◦C), enzyme concentration (>15 g L−1) and reactant ratio (w/w) of sucrose:PHA (>2) did not increase further the rate or yield. The sucrose-functionalized mcl-PHAwas characterized with respect to the non-functionalized material.
CONCLUSIONS: Novozym 435 can be used effectively to synthesize poly(1’-O-3-hydroxyacyl sucrose) in micro-aqueous
medium bypassing the need for chemo-synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches |
| format | Article |
| id | my.um.eprints-9632 |
| institution | Universiti Malaya |
| language | en |
| publishDate | 2013 |
| publisher | Society of Chemical Industry |
| record_format | eprints |
| spelling | my.um.eprints-96322014-04-22T01:51:35Z http://eprints.um.edu.my/9632/ Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates Gumel, A.M. Annuar, M.S.M. Heidelberg, T. Q Science (General) QD Chemistry BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial. RESULTS:Theeffects of reaction variables reaction time, temperature,enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1’-O-3-hydroxyacyl-sucrose) using Candida antarctica lipase B (EC 3.1.1.3)were studied. Using H2O2 as micro-initiator, enzyme-mediated synthesis yielded reaction rate, vapp of 0.076 x 10−5 mol L−1 s−1. The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non-functionalized material apart from showing better compostability. Increasing the reaction temperature (>50◦C), enzyme concentration (>15 g L−1) and reactant ratio (w/w) of sucrose:PHA (>2) did not increase further the rate or yield. The sucrose-functionalized mcl-PHAwas characterized with respect to the non-functionalized material. CONCLUSIONS: Novozym 435 can be used effectively to synthesize poly(1’-O-3-hydroxyacyl sucrose) in micro-aqueous medium bypassing the need for chemo-synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches Society of Chemical Industry 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9632/1/00013161_103461.pdf Gumel, A.M. and Annuar, M.S.M. and Heidelberg, T. (2013) Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates. Journal of Chemical Technology and Biotechnology. pp. 1328-1335. DOI https://doi.org/10.1002/jctb.3980 <https://doi.org/10.1002/jctb.3980>. DOI 10.1002/jctb.3980 |
| spellingShingle | Q Science (General) QD Chemistry Gumel, A.M. Annuar, M.S.M. Heidelberg, T. Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| title | Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| title_full | Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| title_fullStr | Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| title_full_unstemmed | Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| title_short | Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| title_sort | single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
| topic | Q Science (General) QD Chemistry |
| url | http://eprints.um.edu.my/9632/1/00013161_103461.pdf http://eprints.um.edu.my/9632/ |
| url_provider | http://eprints.um.edu.my/ |