Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway
Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Cioc...
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Hindawi
2011
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| Online Access: | http://eprints.um.edu.my/89/ https://doi.org/10.1155/2011/156765 |
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| author | Abdelwahab, Siddig Ibrahim Mohan, Syam Elhassan, Manal Mohamed Al-Mekhlafi, Nabil Mariod, Abdelbasit Adam Abdul, Ahmad Bustamam Abdulla, Mahmood Ameen Alkharfy, Khalid M. |
| author_facet | Abdelwahab, Siddig Ibrahim Mohan, Syam Elhassan, Manal Mohamed Al-Mekhlafi, Nabil Mariod, Abdelbasit Adam Abdul, Ahmad Bustamam Abdulla, Mahmood Ameen Alkharfy, Khalid M. |
| author_sort | Abdelwahab, Siddig Ibrahim |
| building | UM Library |
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| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH. The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR. Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively. Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF. The IC(50) values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively. OSreduced the oxidation of β-carotene by hydroperoxides. Cell death was dose-dependently inhibited by pretreatment with OS. Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS. This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H(2)O(2)-induced apoptosis. The current results showed that phenolics may provide health and nutritional benefits. |
| format | Article |
| id | my.um.eprints-89 |
| institution | Universiti Malaya |
| publishDate | 2011 |
| publisher | Hindawi |
| record_format | eprints |
| spelling | my.um.eprints-892025-04-11T01:41:47Z http://eprints.um.edu.my/89/ Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway Abdelwahab, Siddig Ibrahim Mohan, Syam Elhassan, Manal Mohamed Al-Mekhlafi, Nabil Mariod, Abdelbasit Adam Abdul, Ahmad Bustamam Abdulla, Mahmood Ameen Alkharfy, Khalid M. R Medicine (General) Antiapoptotic and antioxidant activities of aqueous-methanolic extract (CAME) of Orthosiphonstamineus Benth(OS), and its hexane (HF), chloroform (CF), n-butanol (NBF), ethyl acetate (EAF) and water (WF) fractions were investigated. Antioxidant properties were evaluated using the assays of Folin-Ciocalteu, aluminiumtrichloride, β-carotene bleaching and DPPH. The role of OS against hydrogen peroxide induced apoptosis on MDA-M231 epithelial cells was examined using MTT assay, phase contrast microscope, colorimetric assay of caspase-3, western blot and quantitative real-time PCR. Results showed that EAF showed the highest total phenolic content followed by CAME, NBF, WF, CF and HF, respectively. Flavonoid content was in the order of the CF > EAF > HF > CAME > NBF > WF. The IC(50) values on DPPH assay for different extract/fractions were 126.2 ± 23, 31.25 ± 1.2, 15.25 ± 2.3, 13.56 ± 1.9, 23.0 ± 3.2, and 16.66 ± 1.5 μg/ml for HF, CF, EAF, NBF, WF and CAME, respectively. OSreduced the oxidation of β-carotene by hydroperoxides. Cell death was dose-dependently inhibited by pretreatment with OS. Caspase-3 and distinct morphological features suggest the anti-apoptotic activities of OS. This plant not only increased the expression of Bcl-2, but also decreased Bax expression, and ultimately reduced H(2)O(2)-induced apoptosis. The current results showed that phenolics may provide health and nutritional benefits. Hindawi 2011 Article PeerReviewed Abdelwahab, Siddig Ibrahim and Mohan, Syam and Elhassan, Manal Mohamed and Al-Mekhlafi, Nabil and Mariod, Abdelbasit Adam and Abdul, Ahmad Bustamam and Abdulla, Mahmood Ameen and Alkharfy, Khalid M. (2011) Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway. Evidence-Based Complementary and Alternative Medicine, 2011. p. 156765. ISSN 1741-427X, DOI 3018659. https://doi.org/10.1155/2011/156765 3018659 |
| spellingShingle | R Medicine (General) Abdelwahab, Siddig Ibrahim Mohan, Syam Elhassan, Manal Mohamed Al-Mekhlafi, Nabil Mariod, Abdelbasit Adam Abdul, Ahmad Bustamam Abdulla, Mahmood Ameen Alkharfy, Khalid M. Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| title | Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| title_full | Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| title_fullStr | Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| title_full_unstemmed | Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| title_short | Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway |
| title_sort | antiapoptotic and antioxidant properties of orthosiphon stamineus benth (cat's whiskers): intervention in the bcl-2-mediated apoptotic pathway |
| topic | R Medicine (General) |
| url | http://eprints.um.edu.my/89/ https://doi.org/10.1155/2011/156765 |
| url_provider | http://eprints.um.edu.my/ |