Gender identification of mouse embryos using the polymerase chain reaction (PCR) technique
A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three b...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
2006
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/8388/ http://www.indmedica.com/journals.php?journalid=12&issueid=112&articleid=1529&action=article |
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| Summary: | A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three bands, of sizes 217 bp (Zfy), 147 bp (Sry) and 111 bp (Nds). In contrast, amplification of female DNA gave a single band of 111 bp (Nds). Test on blood DNA showed a 98.0% PCR amplification efficiency whereas single blastomeres produced 93.0% PCR amplification efficiency. In conclusion, mouse embryos were successfully sexed within 6 hours by using single blastomeres obtained from 8-cell embryos. |
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