Prevalence and Characterization of Multidrug-Resistant and Extended-Spectrum Beta-Lactamase-Producing Escherichia coli from Pediatric Wards of a Malaysian Hospital
The emergence of Escherichia coli resistant to extended-spectrum cephalosporins (ESCs) is of concern as ESC is often used to treat infections by Gram-negative bacteria. One-hundred and ten E. coli strains isolated in 2009-2010 from children warded in a Malaysian tertiary hospital were analyzed for t...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Mary Ann Liebert
2012
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/5605/1/Ho_et_al_%25282012%2529_Microb_Drug_Resist.pdf http://eprints.um.edu.my/5605/ |
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| Summary: | The emergence of Escherichia coli resistant to extended-spectrum cephalosporins (ESCs) is of concern as ESC is often used to treat infections by Gram-negative bacteria. One-hundred and ten E. coli strains isolated in 2009-2010 from children warded in a Malaysian tertiary hospital were analyzed for their antibiograms, carriage of extended-spectrum beta-lactamase (ESBL) and AmpC genes, possible inclusion of the beta-lactamase genes on an integron platform, and their genetic relatedness. All E. coli strains were sensitive to carbapenems. About 46 of strains were multidrug resistant (MDR; i.e., resistant to >= 3 antibiotic classes) and almost half (45) were nonsusceptible to ESCs. Among the MDR strains, high resistance rates were observed for ampicillin (98), tetracycline (75), and trimethoprim/sulfamethoxazole (73). Out of 110 strains, bla(TEM-1) (49.1), bla(CTX-M) (11.8), and bla(CMY-2) (6.4) were detected. Twenty-one strains were ESBL producers. CTX-M-15 was the predominant CTX-M variant found and this is the first report of a CTX-M-27-producing E. coli strain from Malaysia. Majority (3.1) of the strains harbored class 1 integron-encoded integrases with a predominance of aadA and dfr genes within the integron variable region. No gene cassette encoding ESBL genes was found and integrons were not significantly associated with ESBL or non-ESBL producers. Possible clonal expansion was observed for few CTX-M-15-positive strains but the O25-ST131 E. coli clone known to harbor CTX-M-15 was not detected while CMY-2-positive strains were genetically diverse. |
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