Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan
The objective of this study was to determine the genetic diversity and virulence factors of Vibrio cholerae and V. parahaemolyticus from selected aquatic environments in Bachok, Kelantan, Malaysia. Out of 50 water samples, 33 were confirmed with the presence of presumptive V. cholerae and V. parahae...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Faculty of Science, University of Malaya
2010
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/5576/1/Isolation%2C_Detection_and_Genomic_Differentiation_of_Vibrio_cholerae_and_Vibrio_parahaemolyticus_in_Bachok%2C_Kelantan.pdf http://eprints.um.edu.my/5576/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1831447014962888704 |
|---|---|
| author | Vimala, B. Thong, Kwai Lin Chong, V.C. |
| author_facet | Vimala, B. Thong, Kwai Lin Chong, V.C. |
| author_sort | Vimala, B. |
| building | UM Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | The objective of this study was to determine the genetic diversity and virulence factors of Vibrio cholerae and V. parahaemolyticus from selected aquatic environments in Bachok, Kelantan, Malaysia. Out of 50 water samples, 33 were confirmed with the presence of presumptive V. cholerae and V. parahaemolyticus based on biochemical tests. Primers targeting toxR gene specific for V. cholerae (800bp) and V. parahaemolyticus (368bp), tdh (251bp) and trh (250bp) virulence genes were used in the PCR. Results of PCR showed that only 25 isolates of V. parahaemolyticus and 3 of V. cholerae had the toxR gene. None of the V. parahaemolyticus isolates had the trh and tdh genes. Repetitive extragenic palindromic PCR (REP-PCR) was used to determine the relatedness of these strains at the genomic level. Cluster analysis for REP-PCR showed 2 major clusters A-B, with several isolates from different locations grouped together indicating the geographical relatedness due to interconnected waterways. REPPCR showed that the species of Vibrios are genetically diverse. |
| format | Article |
| id | my.um.eprints-5576 |
| institution | Universiti Malaya |
| language | en |
| publishDate | 2010 |
| publisher | Faculty of Science, University of Malaya |
| record_format | eprints |
| spelling | my.um.eprints-55762021-04-27T07:55:17Z http://eprints.um.edu.my/5576/ Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan Vimala, B. Thong, Kwai Lin Chong, V.C. Q Science (General) QR Microbiology The objective of this study was to determine the genetic diversity and virulence factors of Vibrio cholerae and V. parahaemolyticus from selected aquatic environments in Bachok, Kelantan, Malaysia. Out of 50 water samples, 33 were confirmed with the presence of presumptive V. cholerae and V. parahaemolyticus based on biochemical tests. Primers targeting toxR gene specific for V. cholerae (800bp) and V. parahaemolyticus (368bp), tdh (251bp) and trh (250bp) virulence genes were used in the PCR. Results of PCR showed that only 25 isolates of V. parahaemolyticus and 3 of V. cholerae had the toxR gene. None of the V. parahaemolyticus isolates had the trh and tdh genes. Repetitive extragenic palindromic PCR (REP-PCR) was used to determine the relatedness of these strains at the genomic level. Cluster analysis for REP-PCR showed 2 major clusters A-B, with several isolates from different locations grouped together indicating the geographical relatedness due to interconnected waterways. REPPCR showed that the species of Vibrios are genetically diverse. Faculty of Science, University of Malaya 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5576/1/Isolation%2C_Detection_and_Genomic_Differentiation_of_Vibrio_cholerae_and_Vibrio_parahaemolyticus_in_Bachok%2C_Kelantan.pdf Vimala, B. and Thong, Kwai Lin and Chong, V.C. (2010) Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan. Malaysian Journal of Science, 29 (Specia). pp. 1-10. ISSN 1394-3065, |
| spellingShingle | Q Science (General) QR Microbiology Vimala, B. Thong, Kwai Lin Chong, V.C. Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan |
| title | Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan |
| title_full | Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan |
| title_fullStr | Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan |
| title_full_unstemmed | Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan |
| title_short | Isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in Bachok, Kelantan |
| title_sort | isolation, detection and genomic differentiation of vibrio cholerae and vibrio parahaemolyticus in bachok, kelantan |
| topic | Q Science (General) QR Microbiology |
| url | http://eprints.um.edu.my/5576/1/Isolation%2C_Detection_and_Genomic_Differentiation_of_Vibrio_cholerae_and_Vibrio_parahaemolyticus_in_Bachok%2C_Kelantan.pdf http://eprints.um.edu.my/5576/ |
| url_provider | http://eprints.um.edu.my/ |