A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes.
Sex determination of livestock is performed to achieve the objectives of livestock breeding programmes. Techniques for sex determination have evolved from karyotyping to detecting Y-specific antigens and recently to the polymerase chain reaction (PCR), which appears to be the most sensitive, accurat...
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The Society for Reproduction and Development
2003
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| Online Access: | http://eprints.um.edu.my/488/ https://doi.org/10.1262/jrd.49.307 |
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| author | Phua, Alice Choon Yen Abdullah, Ramli Bin Mohamed, Zulqarnain |
| author_facet | Phua, Alice Choon Yen Abdullah, Ramli Bin Mohamed, Zulqarnain |
| author_sort | Phua, Alice Choon Yen |
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| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | Sex determination of livestock is performed to achieve the objectives of livestock breeding programmes. Techniques for sex determination have evolved from karyotyping to detecting Y-specific antigens and recently to the polymerase chain reaction (PCR), which appears to be the most sensitive, accurate, rapid and reliable method to date. In this study, a PCR-based sex determination method for potential application in goat breeding programmes was developed. Primers were designed to amplify a portion of the X amelogenin gene (Aml-X) on the X chromosome to give a 300 bp product and Sry gene on the Y chromosome to give a 116 bp product. PCR optimization was performed using DNA template extracted from a whole blood sample of Jermasia goats (German Fawn x Katjang) of both sexes. It was possible to identify the sex chromosomes by amplifying both male- and female-specific genes simultaneously in a duplex reaction with males yielding two bands and females yielding one band. The Aml-X primer set, which served as an internal control primer, did not interfere with amplification of the Y-specific sequence even when a low amount of DNA (1 ng) was used. The duplex reaction subjected to a blind test showed 100% (14/14) concordance, proving its accuracy and reliability. The primer sets used were found to be highly specific and were suitable for gender selection of goats. |
| format | Article |
| id | my.um.eprints-488 |
| institution | Universiti Malaya |
| publishDate | 2003 |
| publisher | The Society for Reproduction and Development |
| record_format | eprints |
| spelling | my.um.eprints-4882019-08-02T10:46:31Z http://eprints.um.edu.my/488/ A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. Phua, Alice Choon Yen Abdullah, Ramli Bin Mohamed, Zulqarnain R Medicine (General) Sex determination of livestock is performed to achieve the objectives of livestock breeding programmes. Techniques for sex determination have evolved from karyotyping to detecting Y-specific antigens and recently to the polymerase chain reaction (PCR), which appears to be the most sensitive, accurate, rapid and reliable method to date. In this study, a PCR-based sex determination method for potential application in goat breeding programmes was developed. Primers were designed to amplify a portion of the X amelogenin gene (Aml-X) on the X chromosome to give a 300 bp product and Sry gene on the Y chromosome to give a 116 bp product. PCR optimization was performed using DNA template extracted from a whole blood sample of Jermasia goats (German Fawn x Katjang) of both sexes. It was possible to identify the sex chromosomes by amplifying both male- and female-specific genes simultaneously in a duplex reaction with males yielding two bands and females yielding one band. The Aml-X primer set, which served as an internal control primer, did not interfere with amplification of the Y-specific sequence even when a low amount of DNA (1 ng) was used. The duplex reaction subjected to a blind test showed 100% (14/14) concordance, proving its accuracy and reliability. The primer sets used were found to be highly specific and were suitable for gender selection of goats. The Society for Reproduction and Development 2003-08 Article PeerReviewed Phua, Alice Choon Yen and Abdullah, Ramli Bin and Mohamed, Zulqarnain (2003) A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. Journal of Reproduction and Development, 49 (4). pp. 307-311. ISSN 0916-8818, DOI https://doi.org/10.1262/jrd.49.307 <https://doi.org/10.1262/jrd.49.307>. https://doi.org/10.1262/jrd.49.307 doi:10.1262/jrd.49.307 |
| spellingShingle | R Medicine (General) Phua, Alice Choon Yen Abdullah, Ramli Bin Mohamed, Zulqarnain A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. |
| title | A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. |
| title_full | A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. |
| title_fullStr | A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. |
| title_full_unstemmed | A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. |
| title_short | A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. |
| title_sort | pcr-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the sry and aml-x genes. |
| topic | R Medicine (General) |
| url | http://eprints.um.edu.my/488/ https://doi.org/10.1262/jrd.49.307 |
| url_provider | http://eprints.um.edu.my/ |