Fluoroquinolone resistance in clinical isolates of Streptococcus pneumoniae from Asian countries: ANSORP study
Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC : 4 mug/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinol...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
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Mary Ann Liebert
2004
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| Online Access: | http://eprints.um.edu.my/4582/ |
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| Summary: | Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC : 4 mug/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 —> Val in parE was the most common mutation. Data suggest that Lys137 —> Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 —> Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 —> Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 —> Val and Asn423 —> Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain(23)F-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone. |
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