Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract
Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. viola...
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2012
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| Online Access: | http://eprints.um.edu.my/3377/1/10.pdf http://eprints.um.edu.my/3377/ https://doi.org/10.3390/s120404016 |
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| author | Krishnan, T. Yin, W.F. Chan, Kok Gan |
| author_facet | Krishnan, T. Yin, W.F. Chan, Kok Gan |
| author_sort | Krishnan, T. |
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| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs. |
| format | Article |
| id | my.um.eprints-3377 |
| institution | Universiti Malaya |
| language | en |
| publishDate | 2012 |
| publisher | MDPI |
| record_format | eprints |
| spelling | my.um.eprints-33772018-10-30T01:57:16Z http://eprints.um.edu.my/3377/ Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract Krishnan, T. Yin, W.F. Chan, Kok Gan QH Natural history R Medicine Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs. MDPI 2012 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3377/1/10.pdf Krishnan, T. and Yin, W.F. and Chan, Kok Gan (2012) Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract. Sensors, 12 (4). pp. 4016-4030. ISSN 1424-8220, DOI https://doi.org/10.3390/s120404016 <https://doi.org/10.3390/s120404016>. https://doi.org/10.3390/s120404016 doi:10.3390/s120404016 |
| spellingShingle | QH Natural history R Medicine Krishnan, T. Yin, W.F. Chan, Kok Gan Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract |
| title | Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract |
| title_full | Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract |
| title_fullStr | Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract |
| title_full_unstemmed | Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract |
| title_short | Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract |
| title_sort | inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa pao1 by ayurveda spice clove (syzygium aromaticum) bud extract |
| topic | QH Natural history R Medicine |
| url | http://eprints.um.edu.my/3377/1/10.pdf http://eprints.um.edu.my/3377/ https://doi.org/10.3390/s120404016 |
| url_provider | http://eprints.um.edu.my/ |