Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro
Tissue culture studies of Celosia cristata were established from various explants and the effects of various hormones on morphogenesis of this species were examined. It was found that complete plant regeneration occurred at highest percentage on MS medium supplemented with 2.0 mg/L NAA and 1.5 mg/L...
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Hindawi Publishing Corporation
2012
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| Online Access: | http://eprints.um.edu.my/3190/ http://dx.doi.org/10.1100/2012/359413 |
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| author | Taha, Rosna Mat Wafa, Sharifah Nurashikin |
| author_facet | Taha, Rosna Mat Wafa, Sharifah Nurashikin |
| author_sort | Taha, Rosna Mat |
| building | UM Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | Tissue culture studies of Celosia cristata were established from various explants and the effects of various hormones on morphogenesis of this species were examined. It was found that complete plant regeneration occurred at highest percentage on MS medium supplemented with 2.0 mg/L NAA and 1.5 mg/L BAP, with the best response showed by shoot explants. In vitro flowering was observed on MS basal medium after six weeks. The occurrence of somaclonal variation and changes in cellular behavior from in vivo and in vitro grown plants were investigated through cytological studies and image analysis. It was observed that Mitotic Index (MI), mean chromosome numbers, and mean nuclear to cell area ratio of in vitro root meristem cells were slightly higher compared to in vivo values. However, in vitro plants produced lower mean cell areas but higher nuclear areas when compared to in vivo plants. Thus, no occurrence of somaclonal variation was detected, and this was supported by morphological features of the in vitro plants. |
| format | Article |
| id | my.um.eprints-3190 |
| institution | Universiti Malaya |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| record_format | eprints |
| spelling | my.um.eprints-31902018-10-24T04:04:49Z http://eprints.um.edu.my/3190/ Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro Taha, Rosna Mat Wafa, Sharifah Nurashikin Q Science (General) QH Natural history Tissue culture studies of Celosia cristata were established from various explants and the effects of various hormones on morphogenesis of this species were examined. It was found that complete plant regeneration occurred at highest percentage on MS medium supplemented with 2.0 mg/L NAA and 1.5 mg/L BAP, with the best response showed by shoot explants. In vitro flowering was observed on MS basal medium after six weeks. The occurrence of somaclonal variation and changes in cellular behavior from in vivo and in vitro grown plants were investigated through cytological studies and image analysis. It was observed that Mitotic Index (MI), mean chromosome numbers, and mean nuclear to cell area ratio of in vitro root meristem cells were slightly higher compared to in vivo values. However, in vitro plants produced lower mean cell areas but higher nuclear areas when compared to in vivo plants. Thus, no occurrence of somaclonal variation was detected, and this was supported by morphological features of the in vitro plants. Hindawi Publishing Corporation 2012 Article PeerReviewed Taha, Rosna Mat and Wafa, Sharifah Nurashikin (2012) Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro. Scientific World Journal, 2012. p. 359413. ISSN 2356-6140, DOI PMID: 22593677. http://dx.doi.org/10.1100/2012/359413 PMID: 22593677 |
| spellingShingle | Q Science (General) QH Natural history Taha, Rosna Mat Wafa, Sharifah Nurashikin Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| title | Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| title_full | Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| title_fullStr | Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| title_full_unstemmed | Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| title_short | Plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| title_sort | plant regeneration and cellular behaviour studies in celosia cristata grown in vivo and in vitro |
| topic | Q Science (General) QH Natural history |
| url | http://eprints.um.edu.my/3190/ http://dx.doi.org/10.1100/2012/359413 |
| url_provider | http://eprints.um.edu.my/ |