Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide
The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetra...
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2007
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| Online Access: | http://eprints.um.edu.my/3049/1/Susceptibility_of_Enterococcus_faecalis_biofilm_to_antibiotics_and_calcium_hydroxide.pdf http://eprints.um.edu.my/3049/2/161.pdf http://eprints.um.edu.my/3049/ |
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| author | Chai, W.L. Hamimah, H. Cheng Soo, C. Sallam Atiya, A. Abdullah, M. |
| author_facet | Chai, W.L. Hamimah, H. Cheng Soo, C. Sallam Atiya, A. Abdullah, M. |
| author_sort | Chai, W.L. |
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| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetracycline, vancomycin, vancomycin followed by gentamicin, Ca(OH)2, and phosphate-buffered saline (control). After 1 h of exposure, the antimicrobial activity was neutralized by washing each disc five times in PBS, and then the colony-forming units of the remaining viable bacteria on each disc were counted. The results revealed that only erythromycin, oxytetracycline and Ca(OH)2 showed 100 biofilm kill. An ANOVA with a Bonferroni post hoc test (P < 0.05) detected significant differences among the test agents, except in the ampicillin group versus the co-trimoxazole group. It is concluded that erythromycin, oxytetracycline and Ca(OH)2 are 100 effective in eliminating E. faecalis biofilm, whereas ampicillin, co-trimoxazole, vancomycin, and vancomycin followed by gentamicin are ineffective. |
| format | Article |
| id | my.um.eprints-3049 |
| institution | Universiti Malaya |
| language | en en |
| publishDate | 2007 |
| record_format | eprints |
| spelling | my.um.eprints-30492014-10-27T07:22:16Z http://eprints.um.edu.my/3049/ Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide Chai, W.L. Hamimah, H. Cheng Soo, C. Sallam Atiya, A. Abdullah, M. RK Dentistry The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetracycline, vancomycin, vancomycin followed by gentamicin, Ca(OH)2, and phosphate-buffered saline (control). After 1 h of exposure, the antimicrobial activity was neutralized by washing each disc five times in PBS, and then the colony-forming units of the remaining viable bacteria on each disc were counted. The results revealed that only erythromycin, oxytetracycline and Ca(OH)2 showed 100 biofilm kill. An ANOVA with a Bonferroni post hoc test (P < 0.05) detected significant differences among the test agents, except in the ampicillin group versus the co-trimoxazole group. It is concluded that erythromycin, oxytetracycline and Ca(OH)2 are 100 effective in eliminating E. faecalis biofilm, whereas ampicillin, co-trimoxazole, vancomycin, and vancomycin followed by gentamicin are ineffective. 2007 Article PeerReviewed application/pdf en http://eprints.um.edu.my/3049/1/Susceptibility_of_Enterococcus_faecalis_biofilm_to_antibiotics_and_calcium_hydroxide.pdf application/pdf en http://eprints.um.edu.my/3049/2/161.pdf Chai, W.L. and Hamimah, H. and Cheng Soo, C. and Sallam Atiya, A. and Abdullah, M. (2007) Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide. Journal of Oral Science, 49 (2). pp. 161-166. ISSN 1343-4934, DOI https://doi.org/10.2334/josnusd.49.161 <https://doi.org/10.2334/josnusd.49.161>. 10.2334/josnusd.49.161 |
| spellingShingle | RK Dentistry Chai, W.L. Hamimah, H. Cheng Soo, C. Sallam Atiya, A. Abdullah, M. Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| title | Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| title_full | Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| title_fullStr | Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| title_full_unstemmed | Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| title_short | Susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| title_sort | susceptibility of enterococcus faecalis biofilm to antibiotics and calcium hydroxide |
| topic | RK Dentistry |
| url | http://eprints.um.edu.my/3049/1/Susceptibility_of_Enterococcus_faecalis_biofilm_to_antibiotics_and_calcium_hydroxide.pdf http://eprints.um.edu.my/3049/2/161.pdf http://eprints.um.edu.my/3049/ |
| url_provider | http://eprints.um.edu.my/ |