A simple adherence test for detection of IgM antibodies in typhoid
A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typh...
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Microbiology Society
1989
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| Online Access: | http://eprints.um.edu.my/246/ http://www.ncbi.nlm.nih.gov/pubmed/2473209 |
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| author | Ong, L.Y. Pang, T. Lim, S.H. Tan, E.L. Puthucheary, S.D. |
| author_facet | Ong, L.Y. Pang, T. Lim, S.H. Tan, E.L. Puthucheary, S.D. |
| author_sort | Ong, L.Y. |
| building | UM Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed. |
| format | Article |
| id | my.um.eprints-246 |
| institution | Universiti Malaya |
| publishDate | 1989 |
| publisher | Microbiology Society |
| record_format | eprints |
| spelling | my.um.eprints-2462019-04-18T07:57:40Z http://eprints.um.edu.my/246/ A simple adherence test for detection of IgM antibodies in typhoid Ong, L.Y. Pang, T. Lim, S.H. Tan, E.L. Puthucheary, S.D. R Medicine (General) A simple adherence test to detect IgM antibodies in patients with typhoid is described. The test utilises the IgM-"capture" approach, in which the test serum is applied to microtitration plate wells previously coated with anti-human IgM, followed by application of a stained Salmonella typhi antigen suspension which shows adherence in positive cases. By this test, 58 (95%) of 61 sera from confirmed cases of typhoid possessed IgM antibodies to the H or O or both antigens of S. typhi. In patients for whom a diagnosis of typhoid was based only on a significant Widal-test titre, 31 (41%) of 76 sera had IgM antibodies to the H or O or both antigens of S. typhi. Some cross-reactivity of the IgM antibodies was detected, especially with the O antigens of S. paratyphi A and B. A total of 82 sera from non-typhoidal fevers (leptospirosis, typhus, dengue fever) showed no reactivity in this test. In normal sera there was no detectable IgM to the O antigen of S. typhi and only a small number (3.9%) had low levels of IgM to the H antigen. The significance and potential importance of this simple, sensitive, specific and economical test is discussed. Microbiology Society 1989-07 Article PeerReviewed Ong, L.Y. and Pang, T. and Lim, S.H. and Tan, E.L. and Puthucheary, S.D. (1989) A simple adherence test for detection of IgM antibodies in typhoid. Journal of Medical Microbiology, 29 (3). pp. 195-8. ISSN 0022-2615, DOI 2473209. http://www.ncbi.nlm.nih.gov/pubmed/2473209 2473209 |
| spellingShingle | R Medicine (General) Ong, L.Y. Pang, T. Lim, S.H. Tan, E.L. Puthucheary, S.D. A simple adherence test for detection of IgM antibodies in typhoid |
| title | A simple adherence test for detection of IgM antibodies in typhoid |
| title_full | A simple adherence test for detection of IgM antibodies in typhoid |
| title_fullStr | A simple adherence test for detection of IgM antibodies in typhoid |
| title_full_unstemmed | A simple adherence test for detection of IgM antibodies in typhoid |
| title_short | A simple adherence test for detection of IgM antibodies in typhoid |
| title_sort | simple adherence test for detection of igm antibodies in typhoid |
| topic | R Medicine (General) |
| url | http://eprints.um.edu.my/246/ http://www.ncbi.nlm.nih.gov/pubmed/2473209 |
| url_provider | http://eprints.um.edu.my/ |