Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane
In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), alga...
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2019
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| Online Access: | http://eprints.um.edu.my/23849/ https://doi.org/10.1016/j.ijbiomac.2019.03.235 |
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| author | Ng, I-Son Tang, Malcolm Siong Yii Show, Pau Loke Chiou, Zih Ming Tsai, Jung Chin Chang, Yu Kaung |
| author_facet | Ng, I-Son Tang, Malcolm Siong Yii Show, Pau Loke Chiou, Zih Ming Tsai, Jung Chin Chang, Yu Kaung |
| author_sort | Ng, I-Son |
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| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10 −2 –2.04 × 10 −1 mL/min·cm 2 ) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step. © 2019 Elsevier B.V. |
| format | Article |
| id | my.um.eprints-23849 |
| institution | Universiti Malaya |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | eprints |
| spelling | my.um.eprints-238492020-02-20T00:59:56Z http://eprints.um.edu.my/23849/ Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane Ng, I-Son Tang, Malcolm Siong Yii Show, Pau Loke Chiou, Zih Ming Tsai, Jung Chin Chang, Yu Kaung QC Physics QR Microbiology TP Chemical technology In this work, a chitosan-modified nanofiber membrane was fabricated and used to examine the permeation characteristics of C-phycocyanin (CPC) obtained from Spirulina platensis. The effects of NaCl concentration (0.1–1.0 M), chitosan coupling pH (6–8), chitosan coupling concentration (0.1–3.0%), algal solution pH (6–8), algal mass concentration (0.1–1.0% dw/v), and membrane flux (4.08 × 10 −2 –2.04 × 10 −1 mL/min·cm 2 ) on the penetration performance of the membrane for CPC were investigated. The results show that the order of binding selectivity of the membrane for these proteins is contaminating proteins (TP) > allophycocyanin (APC) > CPC. TP and APC molecules were more easily adsorbed by the chitosan-modified membrane, and the CPC molecules most easily penetrated the membrane without being adsorbed, enhancing CPC purity. The purification factor and total mass flux were 3.3 fold and 66%, respectively, in a single step. © 2019 Elsevier B.V. Elsevier 2019 Article PeerReviewed Ng, I-Son and Tang, Malcolm Siong Yii and Show, Pau Loke and Chiou, Zih Ming and Tsai, Jung Chin and Chang, Yu Kaung (2019) Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane. International Journal of Biological Macromolecules, 132. pp. 615-628. ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2019.03.235 <https://doi.org/10.1016/j.ijbiomac.2019.03.235>. https://doi.org/10.1016/j.ijbiomac.2019.03.235 doi:10.1016/j.ijbiomac.2019.03.235 |
| spellingShingle | QC Physics QR Microbiology TP Chemical technology Ng, I-Son Tang, Malcolm Siong Yii Show, Pau Loke Chiou, Zih Ming Tsai, Jung Chin Chang, Yu Kaung Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| title | Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| title_full | Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| title_fullStr | Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| title_full_unstemmed | Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| title_short | Enhancement of C-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| title_sort | enhancement of c-phycocyanin purity using negative chromatography with chitosan-modified nanofiber membrane |
| topic | QC Physics QR Microbiology TP Chemical technology |
| url | http://eprints.um.edu.my/23849/ https://doi.org/10.1016/j.ijbiomac.2019.03.235 |
| url_provider | http://eprints.um.edu.my/ |