Measurement of binding strength between prey proteins interacting with Toxoplasma gondii SAG1 and SAG2 using isothermal titration calorimetry (ITC)
Following the outcome from a previously performed yeast two-hybrid experiment, the binding strength between T. gondii SAG1 and SAG2 and their respective prey proteins were further confirmed in this study. The sag1, sag2 and their prey genes were amplified and cloned into a pGEMT vector. To express t...
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| Main Authors: | , |
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| Format: | Article |
| Published: |
Springer Verlag (Germany)
2018
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/21560/ https://doi.org/10.1515/ap-2018-0012 |
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