Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71
Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with imm...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Wiley
2018
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/21125/ https://doi.org/10.1002/jmv.25061 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection. |
|---|