Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells
BACKGROUND: Petroselinum crispum (English parsley) is a common herb of the Apiaceae family that is cultivated throughout the world and is widely used as a seasoning condiment. Studies have shown its potential as a medicinal herb. In this study, P. crispum leaf and stem extracts were evaluated for th...
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Wiley
2015
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| Online Access: | http://eprints.um.edu.my/19599/ http://dx.doi.org/10.1002/jsfa.7078 |
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| author | Tang, Esther Lai-Har Rajarajeswaran, Jayakumar Fung, Shin Yee Kanthimathi, M S Subramaniam |
| author_facet | Tang, Esther Lai-Har Rajarajeswaran, Jayakumar Fung, Shin Yee Kanthimathi, M S Subramaniam |
| author_sort | Tang, Esther Lai-Har |
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| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | BACKGROUND: Petroselinum crispum (English parsley) is a common herb of the Apiaceae family that is cultivated throughout the world and is widely used as a seasoning condiment. Studies have shown its potential as a medicinal herb. In this study, P. crispum leaf and stem extracts were evaluated for their antioxidant properties, protection against DNA damage in normal 3T3-L1 cells, and the inhibition of proliferation and migration of the MCF-7 cells. RESULTS: The dichloromethane extract of P. crispum exhibited the highest phenolic content (42.31 ± 0.50 mg GAE g-1) and ferric reducing ability (0.360 ± 0.009 mmol g-1) of the various extractions performed. The extract showed DPPH radical scavenging activity with an IC50 value of 3310.0 ± 80.5 μg mL-1. Mouse fibroblasts (3T3-L1) pre-treated with 400 μg mL-1 of the extract showed 50.9% protection against H2O2-induced DNA damage, suggesting its potential in cancer prevention. The extract (300 μg mL-1) inhibited H2O2-induced MCF-7 cell migration by 41% ± 4%. As cell migration is necessary for metastasis of cancer cells, inhibition of migration is an indication of protection against metastasis. CONCLUSION: Petroselinum crispum has health-promoting properties with the potential to prevent oxidative stress-related diseases and can be developed into functional food. |
| format | Article |
| id | my.um.eprints-19599 |
| institution | Universiti Malaya |
| publishDate | 2015 |
| publisher | Wiley |
| record_format | eprints |
| spelling | my.um.eprints-195992019-03-21T06:32:54Z http://eprints.um.edu.my/19599/ Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells Tang, Esther Lai-Har Rajarajeswaran, Jayakumar Fung, Shin Yee Kanthimathi, M S Subramaniam R Medicine BACKGROUND: Petroselinum crispum (English parsley) is a common herb of the Apiaceae family that is cultivated throughout the world and is widely used as a seasoning condiment. Studies have shown its potential as a medicinal herb. In this study, P. crispum leaf and stem extracts were evaluated for their antioxidant properties, protection against DNA damage in normal 3T3-L1 cells, and the inhibition of proliferation and migration of the MCF-7 cells. RESULTS: The dichloromethane extract of P. crispum exhibited the highest phenolic content (42.31 ± 0.50 mg GAE g-1) and ferric reducing ability (0.360 ± 0.009 mmol g-1) of the various extractions performed. The extract showed DPPH radical scavenging activity with an IC50 value of 3310.0 ± 80.5 μg mL-1. Mouse fibroblasts (3T3-L1) pre-treated with 400 μg mL-1 of the extract showed 50.9% protection against H2O2-induced DNA damage, suggesting its potential in cancer prevention. The extract (300 μg mL-1) inhibited H2O2-induced MCF-7 cell migration by 41% ± 4%. As cell migration is necessary for metastasis of cancer cells, inhibition of migration is an indication of protection against metastasis. CONCLUSION: Petroselinum crispum has health-promoting properties with the potential to prevent oxidative stress-related diseases and can be developed into functional food. Wiley 2015 Article PeerReviewed Tang, Esther Lai-Har and Rajarajeswaran, Jayakumar and Fung, Shin Yee and Kanthimathi, M S Subramaniam (2015) Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells. Journal of the Science of Food and Agriculture, 95 (13). pp. 2763-2771. ISSN 0022-5142, DOI https://doi.org/10.1002/jsfa.7078 <https://doi.org/10.1002/jsfa.7078>. http://dx.doi.org/10.1002/jsfa.7078 doi:10.1002/jsfa.7078 |
| spellingShingle | R Medicine Tang, Esther Lai-Har Rajarajeswaran, Jayakumar Fung, Shin Yee Kanthimathi, M S Subramaniam Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells |
| title | Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells |
| title_full | Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells |
| title_fullStr | Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells |
| title_full_unstemmed | Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells |
| title_short | Petroselinum crispumhas antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells |
| title_sort | petroselinum crispumhas antioxidant properties, protects against dna damage and inhibits proliferation and migration of cancer cells |
| topic | R Medicine |
| url | http://eprints.um.edu.my/19599/ http://dx.doi.org/10.1002/jsfa.7078 |
| url_provider | http://eprints.um.edu.my/ |