A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity
Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Elsevier
2015
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/19492/ http://dx.doi.org/10.1016/j.saa.2015.05.087 |
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| Summary: | Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV-vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39 μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97 × 104 M-1. Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway. |
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