Isolation and molecular sub typing of Salmonella enterica from chicken, beef and street foods in Malaysia
This study was conducted to determine and identify Salmonella isolated from chicken meat, beef and street food (poultry and beef products) and the genetic diversity of Salmonella by pulsed-field gel electrophoresis (PFGE). Eighty-eight non-repeat Salmonella isolates from 300 food samples were iso...
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| Main Authors: | , |
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| Format: | Article |
| Language: | en |
| Published: |
Academic Journals
2010
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/18919/1/00004462_60309.pdf http://eprints.um.edu.my/18919/ |
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| Summary: | This study was conducted to determine and identify Salmonella isolated from chicken meat, beef and
street food (poultry and beef products) and the genetic diversity of Salmonella by pulsed-field gel
electrophoresis (PFGE). Eighty-eight non-repeat Salmonella isolates from 300 food samples were
isolated and characterized using conventional culture, biochemical and serological methods and
confirmation of Salmonella was determined by polymerase chain reaction (PCR) using specific primers.
Among the 88 Salmonella isolates, 11 serovars [Corvallis (n = 33), Typhimurium (n = 18), Hadar (n = 7),
Enteritidis (n = 5), Weltevreden (n = 5), Agona (n = 5), Newport (n = 4), Albany (n = 3), Istanbul (n = 2),
Emek (n = 1) and Wandsworth (n = 1)] were identified. All the 88 Salmonella isolates were typable by
PFGE with 61 distinct pulsed-field profiles. The cluster analysis indicates that 61 different pulsotypes
were separated into groups of identical serovars based on their PFGE profiles. The isolation of various
serovars of Salmonella from different sources indicates the presence and distribution of Salmonella in
raw meats and street foods. Knowledge of variability of subtypes of different serovars and sources of
Salmonella may provide valuable added information for research, risk management, and public health
strategies. |
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