A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains
INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, re...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2017
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/18378/1/A_duplex_endpoint_PCR_assay_for_rapid_detection_and.pdf http://eprints.um.edu.my/18378/ http://dx.doi.org/10.1590/0037-8682-0364-2016 |
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| Summary: | INTRODUCTION:
This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.
METHODS:
Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.
RESULTS:
The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.
CONCLUSIONS:
Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity. |
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