Characterization of salmonella serovars by PCR–single-strand conformation polymorphism analysis
PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR–single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the s...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | en |
| Published: |
American Society for Microbiology
2002
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/15366/1/Characterization_of_Salmonella_Serovars_by_PCR-single-strand_conformation_polymorphism_analysis.pdf http://eprints.um.edu.my/15366/ |
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| Summary: | PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR–single-strand conformation polymorphism
(PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different
Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars
could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella
serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different
Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a
subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella
enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis
and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing
methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity,
simplicity, and typeability. |
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