Callus induction, in vitro plant regeneration and acclimatization of lycium barbarum l. (goji)
Lycium barbarum L. (Goji) belongs to Solanaceae family and native to some areas of China. Ethnobotanical studies has shown that this plant has been consumed by the Chinese since ancient times. It has been used as medicine in providing excellent effects on cardiovascular system and cholesterol le...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | en |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/15050/1/0001.pdf http://eprints.um.edu.my/15050/ |
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| Summary: | Lycium barbarum L. (Goji) belongs to Solanaceae family and native to some areas of
China. Ethnobotanical studies has shown that this plant has been consumed by the Chinese since
ancient times. It has been used as medicine in providing excellent effects on cardiovascular
system and cholesterol level, besides contains high antioxidant and antidiabetic properties. In
the present study, some tissue culture work has been carried out to induce callus, in vitro
regeneration from various explants of Goji and also some acclimatization protocols were
followed to transfer the regenerated plants to soil. The main aims being to establish high efficient
regeneration system for mass production and commercialization for future uses, since the growth
of this species is very limited in Malaysia. The optimum hormonal regime and the most suitable
and responsive explants were identified. Itwas found that leaves and stems gave good responses.
Murashige and Skoog's (MS) medium supplemented with 2.0 mgIL NAA and O.SmgIL BAP
was the best for callus induction and MS media fortified with 1.0 mg/L NAA and 1.0 mg/L BAP
was optimum for in vitro regeneration. The survival rates of plantlets after acclimatization was
63±I.S % on black soil and SO±I.3 % on mixed soil (combination of black and red soil at ratio of
2 to I)), respectively.
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