The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture
Seven different hormone treatments, namely 6-benzylammopunne (BAP) at 2, 3 mg L-1 was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L-1, BAP at 3.3 mg L-1 in combination with IAA at 1.8 and 3.3 mg L-1 and triple combination of BAP at 2.3, IAA at 1.8 and Gibb...
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2008
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| author | Hamad, A.M. Taha, R.M. |
| author_facet | Hamad, A.M. Taha, R.M. |
| author_sort | Hamad, A.M. |
| building | UM Library |
| collection | Institutional Repository |
| content_provider | Universiti Malaya |
| content_source | UM Research Repository |
| continent | Asia |
| country | Malaysia |
| description | Seven different hormone treatments, namely 6-benzylammopunne (BAP) at 2, 3 mg L-1 was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L-1, BAP at 3.3 mg L-1 in combination with IAA at 1.8 and 3.3 mg L-1 and triple combination of BAP at 2.3, IAA at 1.8 and Gibberellic acid (GA3) at 1.0 mg L -1 were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of Smooth cayenne pineapple shoot-tip culture. Combined application of BAP at 3.3 and IAA at 1.8 mg L-1 induced the highest proliferation of 19 shoots/explant and the highest total of 121 and 125 shoots over 4 cycles of multiplication. Raising the IAA to 3.3 mg L-1 resulted in the lowest proliferation and stunted shoots. Incorporation of GA3 improved the shoot length but caused drastic reduction in proliferation. The other treatments showed an intermediate effect. © 2008 Asian Network for Scientific Information. |
| format | Article |
| id | my.um.eprints-10721 |
| institution | Universiti Malaya |
| publishDate | 2008 |
| record_format | eprints |
| spelling | my.um.eprints-107212014-07-21T01:16:38Z http://eprints.um.edu.my/10721/ The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture Hamad, A.M. Taha, R.M. QH301 Biology Seven different hormone treatments, namely 6-benzylammopunne (BAP) at 2, 3 mg L-1 was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L-1, BAP at 3.3 mg L-1 in combination with IAA at 1.8 and 3.3 mg L-1 and triple combination of BAP at 2.3, IAA at 1.8 and Gibberellic acid (GA3) at 1.0 mg L -1 were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of Smooth cayenne pineapple shoot-tip culture. Combined application of BAP at 3.3 and IAA at 1.8 mg L-1 induced the highest proliferation of 19 shoots/explant and the highest total of 121 and 125 shoots over 4 cycles of multiplication. Raising the IAA to 3.3 mg L-1 resulted in the lowest proliferation and stunted shoots. Incorporation of GA3 improved the shoot length but caused drastic reduction in proliferation. The other treatments showed an intermediate effect. © 2008 Asian Network for Scientific Information. 2008 Article PeerReviewed Hamad, A.M. and Taha, R.M. (2008) The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture. Pakistan Journal of Biological Sciences, 11 (3). pp. 386-391. ISSN 1028-8880, |
| spellingShingle | QH301 Biology Hamad, A.M. Taha, R.M. The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture |
| title | The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture |
| title_full | The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture |
| title_fullStr | The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture |
| title_full_unstemmed | The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture |
| title_short | The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture |
| title_sort | effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus i.) merr cv. smooth cayenne) shoot-tip culture |
| topic | QH301 Biology |
| url | http://eprints.um.edu.my/10721/ |
| url_provider | http://eprints.um.edu.my/ |