The characterization of mecA Gene and SCCmec typing in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz
MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA)....
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Universiti Teknologi MARA
2019
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| Online Access: | https://ir.uitm.edu.my/id/eprint/6108/1/6108.pdf https://ir.uitm.edu.my/id/eprint/6108/ https://scilett-fsg.uitm.edu.my/ |
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| author | Aswani, Siti Aisya Abdul-Aziz, Aziyah |
| author_facet | Aswani, Siti Aisya Abdul-Aziz, Aziyah |
| author_sort | Aswani, Siti Aisya |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA). A total of 20 presumptive S. aureus strains were collected from one of the teaching hospitals in Selangor. Using standard biochemical tests, all the isolates were verified as S. aureus. When tested against cefoxitin, 80% of the isolates were confirmed as MRSA. All the MRSA isolates were further subjected to polymerase chain reaction (PCR) to detect the presence of mecA gene. Nine out of the 16 MRSA isolates (56%) were mecA positive, whereas the remaining four were mecA negative. The 16 MRSA with positive mecA gene were further subjected to SCCmec typing of type I, II, III, IV and V. The most frequent SCCmec types were type III (56%) followed by type II (33%), and type IV (11%). None of the isolates were of SCCmec type I or V. Our study indicates that SCCmec type III is predominant among the isolates which is in agreement with other studies conducted on clinical strains of MRSA. |
| format | Article |
| id | my.uitm.ir-6108 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2019 |
| publisher | Universiti Teknologi MARA |
| record_format | eprints |
| spelling | my.uitm.ir-61082021-09-02T04:16:27Z https://ir.uitm.edu.my/id/eprint/6108/ The characterization of mecA Gene and SCCmec typing in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz Aswani, Siti Aisya Abdul-Aziz, Aziyah DNA. Deoxyribonucleic acids Clinical pathology. Laboratory technique MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA). A total of 20 presumptive S. aureus strains were collected from one of the teaching hospitals in Selangor. Using standard biochemical tests, all the isolates were verified as S. aureus. When tested against cefoxitin, 80% of the isolates were confirmed as MRSA. All the MRSA isolates were further subjected to polymerase chain reaction (PCR) to detect the presence of mecA gene. Nine out of the 16 MRSA isolates (56%) were mecA positive, whereas the remaining four were mecA negative. The 16 MRSA with positive mecA gene were further subjected to SCCmec typing of type I, II, III, IV and V. The most frequent SCCmec types were type III (56%) followed by type II (33%), and type IV (11%). None of the isolates were of SCCmec type I or V. Our study indicates that SCCmec type III is predominant among the isolates which is in agreement with other studies conducted on clinical strains of MRSA. Universiti Teknologi MARA 2019-12-06 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/6108/1/6108.pdf The characterization of mecA Gene and SCCmec typing in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz. (2019) Science Letters (ScL) <https://ir.uitm.edu.my/view/publication/Science_Letters_=28ScL=29/>, 13 (2): 7. pp. 1-7. ISSN eISSN: 2682-8626 https://scilett-fsg.uitm.edu.my/ 10.1234/jmpc.v13i2.7525 10.1234/jmpc.v13i2.7525 10.1234/jmpc.v13i2.7525 |
| spellingShingle | DNA. Deoxyribonucleic acids Clinical pathology. Laboratory technique Aswani, Siti Aisya Abdul-Aziz, Aziyah The characterization of mecA Gene and SCCmec typing in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz |
| title | The characterization of mecA Gene and SCCmec typing
in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz |
| title_full | The characterization of mecA Gene and SCCmec typing
in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz |
| title_fullStr | The characterization of mecA Gene and SCCmec typing
in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz |
| title_full_unstemmed | The characterization of mecA Gene and SCCmec typing
in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz |
| title_short | The characterization of mecA Gene and SCCmec typing
in clinical samples of MRSA / Siti Aisya Aswani and Aziyah Abdul-Aziz |
| title_sort | characterization of meca gene and sccmec typing
in clinical samples of mrsa / siti aisya aswani and aziyah abdul-aziz |
| topic | DNA. Deoxyribonucleic acids Clinical pathology. Laboratory technique |
| url | https://ir.uitm.edu.my/id/eprint/6108/1/6108.pdf https://ir.uitm.edu.my/id/eprint/6108/ https://scilett-fsg.uitm.edu.my/ |
| url_provider | http://ir.uitm.edu.my/ |