Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah

Properties of protein hydrolysate prepared from green mussel (Perna viridis) by enzymatic hydrolysis using AlcalaseĀ® 2.4L at different pH and enzyme to substrate ratio were determined. Hydrolysis was performed using the pH-stat method. Mussel hydrolysate produced at pH 7 with E/S 5% had protein cont...

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Main Author: Mohd Shah, Siti Hafsah
Format: Thesis
Language:en
Published: 2012
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Online Access:https://ir.uitm.edu.my/id/eprint/52976/1/52976.pdf
https://ir.uitm.edu.my/id/eprint/52976/
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author Mohd Shah, Siti Hafsah
author_facet Mohd Shah, Siti Hafsah
author_sort Mohd Shah, Siti Hafsah
building Tun Abdul Razak Library
collection Institutional Repository
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
continent Asia
country Malaysia
description Properties of protein hydrolysate prepared from green mussel (Perna viridis) by enzymatic hydrolysis using AlcalaseĀ® 2.4L at different pH and enzyme to substrate ratio were determined. Hydrolysis was performed using the pH-stat method. Mussel hydrolysate produced at pH 7 with E/S 5% had protein content of 37.17%. Mussel hydrolysate produced at pH 7 had low molecular weight peptide which was < 18 kDa. It also contain high amount of Methionine, Phenylalanine and Proline which contributed to bitter taste. Mussel hydrolysate produced at pH 7 was the most acceptable and the degree of bitterness was slightly bitter but not exceeded the bitterness of the standard caffeine. These results suggested that green mussel (Perna viridis) hydrolysates have the potential to be applied as a flavoring agent in the food since the bitterness is still in acceptable range.
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spelling my.uitm.ir-529762021-12-22T06:48:08Z https://ir.uitm.edu.my/id/eprint/52976/ Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah Mohd Shah, Siti Hafsah Biochemistry Properties of protein hydrolysate prepared from green mussel (Perna viridis) by enzymatic hydrolysis using AlcalaseĀ® 2.4L at different pH and enzyme to substrate ratio were determined. Hydrolysis was performed using the pH-stat method. Mussel hydrolysate produced at pH 7 with E/S 5% had protein content of 37.17%. Mussel hydrolysate produced at pH 7 had low molecular weight peptide which was < 18 kDa. It also contain high amount of Methionine, Phenylalanine and Proline which contributed to bitter taste. Mussel hydrolysate produced at pH 7 was the most acceptable and the degree of bitterness was slightly bitter but not exceeded the bitterness of the standard caffeine. These results suggested that green mussel (Perna viridis) hydrolysates have the potential to be applied as a flavoring agent in the food since the bitterness is still in acceptable range. 2012 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/52976/1/52976.pdf Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah. (2012) Degree thesis, thesis, Universiti Teknologi MARA.
spellingShingle Biochemistry
Mohd Shah, Siti Hafsah
Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_full Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_fullStr Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_full_unstemmed Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_short Evaluation on the degree of bitterness of green mussel (Perna viridis) protein hydrolysate / Siti Hafsah Mohd Shah
title_sort evaluation on the degree of bitterness of green mussel (perna viridis) protein hydrolysate / siti hafsah mohd shah
topic Biochemistry
url https://ir.uitm.edu.my/id/eprint/52976/1/52976.pdf
https://ir.uitm.edu.my/id/eprint/52976/
url_provider http://ir.uitm.edu.my/