Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri
Lipase from Candida rugosa was immobilized onto N-vinyl-2-pyyrolidone -co-styrene (VP-co-ST) hydrogel by an entrapment method. The percentage composition of monomer used to synthesizethe VP-co-ST hydrogel is (VP:ST)% ; 10:90 . The VP-co-ST hydrogel immobilized lipase was used to catalyze the enantio...
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| Format: | Conference or Workshop Item |
| Language: | en |
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2004
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| Online Access: | https://ir.uitm.edu.my/id/eprint/51202/1/51202.PDF https://ir.uitm.edu.my/id/eprint/51202/ |
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| author | Harun, Aiza Basri, Mahiran |
| author_facet | Harun, Aiza Basri, Mahiran |
| author_sort | Harun, Aiza |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Lipase from Candida rugosa was immobilized onto N-vinyl-2-pyyrolidone -co-styrene (VP-co-ST) hydrogel by an entrapment method. The percentage composition of monomer used to synthesizethe VP-co-ST hydrogel is (VP:ST)% ; 10:90 . The VP-co-ST hydrogel immobilized lipase was used to catalyze the enantioselective esterification of (R.S)-2-(4-chlorophenoxy) propanoic acid with n-tetradecanol to produce (R)- 2- (4 chlorophenoxy) propanoate and (S) - 2- (4-chJorophenoxy) propanoic acid. The VP-co-ST hydrogel immobilized lipase with the percentage of monomers 10:90 had beed chosen since it exhibited higher enantioselectivity compared with free lipase at optimum time 24 hours. The optimum temperature for the VP-co-ST hydrogel immobilized lipase and free lipase to catalyze the enantioselective esterification is at 40°C whereby the VP-co-ST hydrogel immobilized lipase exhibited higher enantioselectivity (enantiomeric excess) at 45%. The best solvent media for both lipases to catalyze the same reaction when using carbon tetrachloride, in which VP-co-ST hydrogel immobilized lipase showed higher percentage of enantioselectivity that is almost 50% compared to free lipase. |
| format | Conference or Workshop Item |
| id | my.uitm.ir-51202 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2004 |
| record_format | eprints |
| spelling | my.uitm.ir-512022022-09-22T13:49:38Z https://ir.uitm.edu.my/id/eprint/51202/ Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri Harun, Aiza Basri, Mahiran Extraction (Chemistry) Genetics Enzymes Lipase from Candida rugosa was immobilized onto N-vinyl-2-pyyrolidone -co-styrene (VP-co-ST) hydrogel by an entrapment method. The percentage composition of monomer used to synthesizethe VP-co-ST hydrogel is (VP:ST)% ; 10:90 . The VP-co-ST hydrogel immobilized lipase was used to catalyze the enantioselective esterification of (R.S)-2-(4-chlorophenoxy) propanoic acid with n-tetradecanol to produce (R)- 2- (4 chlorophenoxy) propanoate and (S) - 2- (4-chJorophenoxy) propanoic acid. The VP-co-ST hydrogel immobilized lipase with the percentage of monomers 10:90 had beed chosen since it exhibited higher enantioselectivity compared with free lipase at optimum time 24 hours. The optimum temperature for the VP-co-ST hydrogel immobilized lipase and free lipase to catalyze the enantioselective esterification is at 40°C whereby the VP-co-ST hydrogel immobilized lipase exhibited higher enantioselectivity (enantiomeric excess) at 45%. The best solvent media for both lipases to catalyze the same reaction when using carbon tetrachloride, in which VP-co-ST hydrogel immobilized lipase showed higher percentage of enantioselectivity that is almost 50% compared to free lipase. 2004 Conference or Workshop Item PeerReviewed text en https://ir.uitm.edu.my/id/eprint/51202/1/51202.PDF Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri. (2004) In: STSS 2004 : SAINS TEKNOLOGI JILID 1, 31 MEI – 1 JUN 2004, HOTEL VISTANA, KUANTAN, PAHANG. |
| spellingShingle | Extraction (Chemistry) Genetics Enzymes Harun, Aiza Basri, Mahiran Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri |
| title | Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri |
| title_full | Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri |
| title_fullStr | Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri |
| title_full_unstemmed | Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri |
| title_short | Immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / Aiza Harun and Mahiran Basri |
| title_sort | immobilization of candida rugosa lipase onto n-vinyl-2-pyyrolidone-co-styrene for use in enantioselective esterification / aiza harun and mahiran basri |
| topic | Extraction (Chemistry) Genetics Enzymes |
| url | https://ir.uitm.edu.my/id/eprint/51202/1/51202.PDF https://ir.uitm.edu.my/id/eprint/51202/ |
| url_provider | http://ir.uitm.edu.my/ |