Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan
The aims of this study are to isolate bacteria and determine pathogenic bacteria present in edible ice served in drinks and beverages. The consumption of ice cube in drinks and beverages as coolant agent is very common in Kuala Pilah, Negeri Sembilan. Thus, contamination in ice cube may be a vehicle...
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| Format: | Student Project |
| Language: | en |
| Published: |
2017
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| Online Access: | https://ir.uitm.edu.my/id/eprint/33268/1/PPb_WAN%20SHAHIRAH%20W%20ADNAN%20AS%20N%2017_5.pdf https://ir.uitm.edu.my/id/eprint/33268/ |
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| _version_ | 1833064491464523776 |
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| author | W Adnan, Wan Shahirah |
| author_facet | W Adnan, Wan Shahirah |
| author_sort | W Adnan, Wan Shahirah |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The aims of this study are to isolate bacteria and determine pathogenic bacteria present in edible ice served in drinks and beverages. The consumption of ice cube in drinks and beverages as coolant agent is very common in Kuala Pilah, Negeri Sembilan. Thus, contamination in ice cube may be a vehicle in transmission of disease to the public. The ice cube samples were collected randomly from food premises in Pekan Kuala Pilah, Negeri Sembilan. The samples collected filtered using cellulose nitrate membrane (0.45 μm pore size, 47 mm diameter) and proceed with placement of membrane on MacConkey and nutrient agar. Successfully isolated bacteria were tested using IMViC test and gram staining to determine their characteristics. The species and genus of the isolated bacteria were then confirmed with DNA sequencing. Succesfully isolated bacteria were identified as Klebsiella pneumoniae, Acinetobacter baumanii, and Bacillus lichenifromis. Klebsiella pneumoniae were isolated from two out of three samples collected. |
| format | Student Project |
| id | my.uitm.ir-33268 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2017 |
| record_format | eprints |
| spelling | my.uitm.ir-332682020-08-12T09:23:42Z https://ir.uitm.edu.my/id/eprint/33268/ Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan W Adnan, Wan Shahirah Laboratories. General works Bacteria The aims of this study are to isolate bacteria and determine pathogenic bacteria present in edible ice served in drinks and beverages. The consumption of ice cube in drinks and beverages as coolant agent is very common in Kuala Pilah, Negeri Sembilan. Thus, contamination in ice cube may be a vehicle in transmission of disease to the public. The ice cube samples were collected randomly from food premises in Pekan Kuala Pilah, Negeri Sembilan. The samples collected filtered using cellulose nitrate membrane (0.45 μm pore size, 47 mm diameter) and proceed with placement of membrane on MacConkey and nutrient agar. Successfully isolated bacteria were tested using IMViC test and gram staining to determine their characteristics. The species and genus of the isolated bacteria were then confirmed with DNA sequencing. Succesfully isolated bacteria were identified as Klebsiella pneumoniae, Acinetobacter baumanii, and Bacillus lichenifromis. Klebsiella pneumoniae were isolated from two out of three samples collected. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/33268/1/PPb_WAN%20SHAHIRAH%20W%20ADNAN%20AS%20N%2017_5.pdf Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan. (2017) [Student Project] (Unpublished) |
| spellingShingle | Laboratories. General works Bacteria W Adnan, Wan Shahirah Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan |
| title | Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan |
| title_full | Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan |
| title_fullStr | Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan |
| title_full_unstemmed | Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan |
| title_short | Isolated bacteria from ice cubes sampled from food premises in Kuala Pilah, Negeri Sembilan / Wan Shahirah W Adnan |
| title_sort | isolated bacteria from ice cubes sampled from food premises in kuala pilah, negeri sembilan / wan shahirah w adnan |
| topic | Laboratories. General works Bacteria |
| url | https://ir.uitm.edu.my/id/eprint/33268/1/PPb_WAN%20SHAHIRAH%20W%20ADNAN%20AS%20N%2017_5.pdf https://ir.uitm.edu.my/id/eprint/33268/ |
| url_provider | http://ir.uitm.edu.my/ |