Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]

Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family. The objectives of this study were to determine antimicrobial activity and Minimal Inhibition Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Met...

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Main Authors: Nawi, Liliwirianis, Md Isa, Nurun Nadhirah, Musa, Nor Lailatul Wahidah, Mohd Jan, Sarah Laila, Shaikh Nasir, Nur Thabitah
Format: Article
Language:en
Published: Universiti Teknologi MARA Cawangan Pahang 2019
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Online Access:https://ir.uitm.edu.my/id/eprint/31241/1/31241.pdf
https://ir.uitm.edu.my/id/eprint/31241/
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author Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
author_facet Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
author_sort Nawi, Liliwirianis
building Tun Abdul Razak Library
collection Institutional Repository
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
continent Asia
country Malaysia
description Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family. The objectives of this study were to determine antimicrobial activity and Minimal Inhibition Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Methanol and hexane were the two extraction solvents that had been used. Four concentrations of extracts; 50 mg/mL, 100 mg/mL, 200 mg/mL, and 400 mg/mL were prepared for antimicrobial activity. Meanwhile for MIC determination, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL and 50 mg/mL concentrations were prepared. By using disc diffusion method, the methanol extract of G. procumbens leaves showed antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa compared to hexane extract. Highest antimicrobial activities were recorded against S. aureus at 400 mg/mL concentrations with 10.5 mm of inhibition zone. Broth dilution assay resulted MIC for methanol crude extract against S. aureus, B. subtilis, K. pneumoniae, and P. aeruginosa were at 12.5 mg/mL, 25 mg/mL, and 50 mg/mL, respectively
format Article
id my.uitm.ir-31241
institution Universiti Teknologi Mara
language en
publishDate 2019
publisher Universiti Teknologi MARA Cawangan Pahang
record_format eprints
spelling my.uitm.ir-312412020-12-09T07:41:01Z https://ir.uitm.edu.my/id/eprint/31241/ Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.] gadingst Nawi, Liliwirianis Md Isa, Nurun Nadhirah Musa, Nor Lailatul Wahidah Mohd Jan, Sarah Laila Shaikh Nasir, Nur Thabitah Medicinal plants (Culture only) Biotechnology Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family. The objectives of this study were to determine antimicrobial activity and Minimal Inhibition Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Methanol and hexane were the two extraction solvents that had been used. Four concentrations of extracts; 50 mg/mL, 100 mg/mL, 200 mg/mL, and 400 mg/mL were prepared for antimicrobial activity. Meanwhile for MIC determination, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL and 50 mg/mL concentrations were prepared. By using disc diffusion method, the methanol extract of G. procumbens leaves showed antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa compared to hexane extract. Highest antimicrobial activities were recorded against S. aureus at 400 mg/mL concentrations with 10.5 mm of inhibition zone. Broth dilution assay resulted MIC for methanol crude extract against S. aureus, B. subtilis, K. pneumoniae, and P. aeruginosa were at 12.5 mg/mL, 25 mg/mL, and 50 mg/mL, respectively Universiti Teknologi MARA Cawangan Pahang 2019-03-31 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/31241/1/31241.pdf Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]. (2019) Gading Journal for Science and Technology <https://ir.uitm.edu.my/view/publication/Gading_Journal_for_Science_and_Technology/>, 2 (1). pp. 17-22. ISSN 2637-0018
spellingShingle Medicinal plants (Culture only)
Biotechnology
Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_full Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_fullStr Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_full_unstemmed Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_short Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_sort antimicrobial activities of gynura procumbens leaves extract against selected bacteria / liliwirianis nawi ...[et al.]
topic Medicinal plants (Culture only)
Biotechnology
url https://ir.uitm.edu.my/id/eprint/31241/1/31241.pdf
https://ir.uitm.edu.my/id/eprint/31241/
url_provider http://ir.uitm.edu.my/