Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman
Processed foods with Halal label (issued by Islamic Development of Malaysia, JAKIM) were selected randomly to be tested for pork-free authentication. As a reference subject, pork-derived processed foods and several types of raw meats were also tested. The investigation was performed by using PCR-RFL...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Research Reports |
| Language: | en |
| Published: |
2005
|
| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/2838/1/2838.pdf https://ir.uitm.edu.my/id/eprint/2838/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1833058239300763648 |
|---|---|
| author | Mustakim, Maimunah Mohd Noor, Zainon (Prof Madya Dr) Camalxaman, Siti Nazrina |
| author_facet | Mustakim, Maimunah Mohd Noor, Zainon (Prof Madya Dr) Camalxaman, Siti Nazrina |
| author_sort | Mustakim, Maimunah |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Processed foods with Halal label (issued by Islamic Development of Malaysia, JAKIM) were selected randomly to be tested for pork-free authentication. As a reference subject, pork-derived processed foods and several types of raw meats were also tested. The investigation was performed by using PCR-RFLP technique, which detected polymorphisms of the conserved region in the mitochondrial cytochrome b (cyt b) gene segment. PCR-amplified 359 bp of cyt b gene using universal primers, CYTbl and CYTb2 from the food samples was further resolved by eight different types of restriction enzymes, Alul, Hindlll, BsaJl, Rsal, BstNl, Msel, Nsil and BstUl. Application of the PCR-RFLP techniques showed distinct DNA fragments in the individual food samples. However, only certain enzymes showed discrimination amongst the tested food samples. |
| format | Research Reports |
| id | my.uitm.ir-2838 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2005 |
| record_format | eprints |
| spelling | my.uitm.ir-28382021-08-23T05:42:57Z https://ir.uitm.edu.my/id/eprint/2838/ Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman Mustakim, Maimunah Mohd Noor, Zainon (Prof Madya Dr) Camalxaman, Siti Nazrina Analytical chemistry Biotechnology Processed foods with Halal label (issued by Islamic Development of Malaysia, JAKIM) were selected randomly to be tested for pork-free authentication. As a reference subject, pork-derived processed foods and several types of raw meats were also tested. The investigation was performed by using PCR-RFLP technique, which detected polymorphisms of the conserved region in the mitochondrial cytochrome b (cyt b) gene segment. PCR-amplified 359 bp of cyt b gene using universal primers, CYTbl and CYTb2 from the food samples was further resolved by eight different types of restriction enzymes, Alul, Hindlll, BsaJl, Rsal, BstNl, Msel, Nsil and BstUl. Application of the PCR-RFLP techniques showed distinct DNA fragments in the individual food samples. However, only certain enzymes showed discrimination amongst the tested food samples. 2005 Research Reports NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/2838/1/2838.pdf Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman. (2005) [Research Reports] (Unpublished) |
| spellingShingle | Analytical chemistry Biotechnology Mustakim, Maimunah Mohd Noor, Zainon (Prof Madya Dr) Camalxaman, Siti Nazrina Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman |
| title | Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman |
| title_full | Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman |
| title_fullStr | Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman |
| title_full_unstemmed | Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman |
| title_short | Monitoring pork-free food products by Polymerase Chain Reaction (PCR) assay / Maimunah Mustakim, Prof Madya Dr Zainon Mohd Noor and Siti Nazrina Camalxaman |
| title_sort | monitoring pork-free food products by polymerase chain reaction (pcr) assay / maimunah mustakim, prof madya dr zainon mohd noor and siti nazrina camalxaman |
| topic | Analytical chemistry Biotechnology |
| url | https://ir.uitm.edu.my/id/eprint/2838/1/2838.pdf https://ir.uitm.edu.my/id/eprint/2838/ |
| url_provider | http://ir.uitm.edu.my/ |