Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.].
Bacteriophages are viruses that can only infect bacterial cells and are perceptible in almost all places where living bacteria are found, as they are highly host specific. Wastewater comprises microorganisms that have become contaminated by the environment. These microorganisms, particularly bacteri...
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| Format: | Article |
| Language: | en |
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Universiti Teknologi MARA, Negeri Sembilan
2025
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| Online Access: | https://ir.uitm.edu.my/id/eprint/116021/1/116021.pdf https://doi.org/10.24191/joa.v13i1 https://ir.uitm.edu.my/id/eprint/116021/ https://journal.uitm.edu.my/ojs/index.php/JOA |
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| author | Anuar, Muhammad Hafizhullah Zulkifly, Nur Surya Zainal-Abidin, Nurhamimah Syed Mohamad, Sharifah Aminah Hasbullah, Nur Intan Iberahim, Rashidah |
| author_facet | Anuar, Muhammad Hafizhullah Zulkifly, Nur Surya Zainal-Abidin, Nurhamimah Syed Mohamad, Sharifah Aminah Hasbullah, Nur Intan Iberahim, Rashidah |
| author_sort | Anuar, Muhammad Hafizhullah |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Bacteriophages are viruses that can only infect bacterial cells and are perceptible in almost all places where living bacteria are found, as they are highly host specific. Wastewater comprises microorganisms that have become contaminated by the environment. These microorganisms, particularly bacteria, may develop antibiotic resistance and constitute a serious threat to human health. Therefore, the goal of this research is to isolate coliphage from untreated wastewater and to identify the susceptible coliform host. The method used for bacteriophage isolation is plaque formation assay and the selected plaque was further enriched and purified using the double-layer plaque technique to classify bacteriophages. Identification of susceptible coliform host using gram stain and biochemical analysis results were compared with other coliform bacteria (Klebsiella sp, Escherichia coli, and Shigella sp.) to identify the susceptible coliphage host. The isolated coliphages appear late from 2 out of 3 samples collected around Kuala Pilah town. The plaque shows were small and turbid after 7 days of incubation in 37°C. It was suspected to be a temperate phage as the plaque developed was turbid. The susceptible host shows similar biochemical characteristics as Klebsiella sp. In conclusion, the bacteriophage obtained from this study is a temperate bacteriophage from environmental wastewater that could infect a coliphage like Klebsiella sp. Further identification of susceptible host was suggested including determination of its medical usage. |
| format | Article |
| id | my.uitm.ir-116021 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2025 |
| publisher | Universiti Teknologi MARA, Negeri Sembilan |
| record_format | eprints |
| spelling | my.uitm.ir-1160212025-07-08T03:13:57Z https://ir.uitm.edu.my/id/eprint/116021/ Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. joa Anuar, Muhammad Hafizhullah Zulkifly, Nur Surya Zainal-Abidin, Nurhamimah Syed Mohamad, Sharifah Aminah Hasbullah, Nur Intan Iberahim, Rashidah Q Science (General) Biotechnology Bacteriophages are viruses that can only infect bacterial cells and are perceptible in almost all places where living bacteria are found, as they are highly host specific. Wastewater comprises microorganisms that have become contaminated by the environment. These microorganisms, particularly bacteria, may develop antibiotic resistance and constitute a serious threat to human health. Therefore, the goal of this research is to isolate coliphage from untreated wastewater and to identify the susceptible coliform host. The method used for bacteriophage isolation is plaque formation assay and the selected plaque was further enriched and purified using the double-layer plaque technique to classify bacteriophages. Identification of susceptible coliform host using gram stain and biochemical analysis results were compared with other coliform bacteria (Klebsiella sp, Escherichia coli, and Shigella sp.) to identify the susceptible coliphage host. The isolated coliphages appear late from 2 out of 3 samples collected around Kuala Pilah town. The plaque shows were small and turbid after 7 days of incubation in 37°C. It was suspected to be a temperate phage as the plaque developed was turbid. The susceptible host shows similar biochemical characteristics as Klebsiella sp. In conclusion, the bacteriophage obtained from this study is a temperate bacteriophage from environmental wastewater that could infect a coliphage like Klebsiella sp. Further identification of susceptible host was suggested including determination of its medical usage. Universiti Teknologi MARA, Negeri Sembilan 2025-04 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/116021/1/116021.pdf Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. (2025) Journal of Academia <https://ir.uitm.edu.my/view/publication/Journal_of_Academia.html>, 13 (1): 3. pp. 29-33. ISSN 2289-6368 https://journal.uitm.edu.my/ojs/index.php/JOA https://doi.org/10.24191/joa.v13i1 https://doi.org/10.24191/joa.v13i1 |
| spellingShingle | Q Science (General) Biotechnology Anuar, Muhammad Hafizhullah Zulkifly, Nur Surya Zainal-Abidin, Nurhamimah Syed Mohamad, Sharifah Aminah Hasbullah, Nur Intan Iberahim, Rashidah Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. |
| title | Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. |
| title_full | Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. |
| title_fullStr | Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. |
| title_full_unstemmed | Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. |
| title_short | Isolation and identification of coliphage from untreated wastewater in Kuala Pilah Negeri Sembilan / Muhammad Hafizhullah Anuar ... [et al.]. |
| title_sort | isolation and identification of coliphage from untreated wastewater in kuala pilah negeri sembilan / muhammad hafizhullah anuar ... [et al.]. |
| topic | Q Science (General) Biotechnology |
| url | https://ir.uitm.edu.my/id/eprint/116021/1/116021.pdf https://doi.org/10.24191/joa.v13i1 https://ir.uitm.edu.my/id/eprint/116021/ https://journal.uitm.edu.my/ojs/index.php/JOA https://doi.org/10.24191/joa.v13i1 |
| url_provider | http://ir.uitm.edu.my/ |