Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi
The RNase L inhibitor (RLI) is a new type of endoribonuclease inhibitor. RLI cDNA codes for a 68-kDa polypeptide which expression is not regulated by interferon (IFN). Its expression antagonizes the 2-5A binding ability and the nuclease activity of the endogenous RNase L. RLI does not lead to 2-5A d...
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| Format: | Thesis |
| Language: | en |
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2009
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| Online Access: | https://ir.uitm.edu.my/id/eprint/105949/1/105949.PDF https://ir.uitm.edu.my/id/eprint/105949/ |
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| _version_ | 1833322981817843712 |
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| author | Azuratmi, Bibi Norazilah |
| author_facet | Azuratmi, Bibi Norazilah |
| author_sort | Azuratmi, Bibi Norazilah |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The RNase L inhibitor (RLI) is a new type of endoribonuclease inhibitor. RLI cDNA codes for a 68-kDa polypeptide which expression is not regulated by interferon (IFN). Its expression antagonizes the 2-5A binding ability and the nuclease activity of the endogenous RNase L. RLI does not lead to 2-5A degradation or to irreversible modification of RNase L. The aim of this study is to clone and express RLI in E. coli. The RT-PCR method was used to amplify the RLI. A set of primers have been designed to produce the target sequence. Few parameters were adjusted to optimize the RT-PCR for specificity and reproducibility. However due to time constraint, this study fails to amplify and clone the RLI in the E. coli. |
| format | Thesis |
| id | my.uitm.ir-105949 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2009 |
| record_format | eprints |
| spelling | my.uitm.ir-1059492024-12-19T17:02:58Z https://ir.uitm.edu.my/id/eprint/105949/ Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi Azuratmi, Bibi Norazilah Pharmaceutical industry The RNase L inhibitor (RLI) is a new type of endoribonuclease inhibitor. RLI cDNA codes for a 68-kDa polypeptide which expression is not regulated by interferon (IFN). Its expression antagonizes the 2-5A binding ability and the nuclease activity of the endogenous RNase L. RLI does not lead to 2-5A degradation or to irreversible modification of RNase L. The aim of this study is to clone and express RLI in E. coli. The RT-PCR method was used to amplify the RLI. A set of primers have been designed to produce the target sequence. Few parameters were adjusted to optimize the RT-PCR for specificity and reproducibility. However due to time constraint, this study fails to amplify and clone the RLI in the E. coli. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/105949/1/105949.PDF Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi. (2009) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam). |
| spellingShingle | Pharmaceutical industry Azuratmi, Bibi Norazilah Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi |
| title | Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi |
| title_full | Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi |
| title_fullStr | Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi |
| title_full_unstemmed | Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi |
| title_short | Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi |
| title_sort | cloning of rnase l inhibitor (rli) / bibi norazilah azuratmi |
| topic | Pharmaceutical industry |
| url | https://ir.uitm.edu.my/id/eprint/105949/1/105949.PDF https://ir.uitm.edu.my/id/eprint/105949/ |
| url_provider | http://ir.uitm.edu.my/ |