Cloning of RNase L inhibitor (RLI) / Bibi Norazilah Azuratmi
The RNase L inhibitor (RLI) is a new type of endoribonuclease inhibitor. RLI cDNA codes for a 68-kDa polypeptide which expression is not regulated by interferon (IFN). Its expression antagonizes the 2-5A binding ability and the nuclease activity of the endogenous RNase L. RLI does not lead to 2-5A d...
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| Format: | Thesis |
| Language: | en |
| Published: |
2009
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| Online Access: | https://ir.uitm.edu.my/id/eprint/105949/1/105949.PDF https://ir.uitm.edu.my/id/eprint/105949/ |
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| Summary: | The RNase L inhibitor (RLI) is a new type of endoribonuclease inhibitor. RLI cDNA codes for a 68-kDa polypeptide which expression is not regulated by interferon (IFN). Its expression antagonizes the 2-5A binding ability and the nuclease activity of the endogenous RNase L. RLI does not lead to 2-5A degradation or to irreversible modification of RNase L. The aim of this study is to clone and express RLI in E. coli. The RT-PCR method was used to amplify the RLI. A set of primers have been designed to produce the target sequence. Few parameters were adjusted to optimize the RT-PCR for specificity and reproducibility. However due to time constraint, this study fails to amplify and clone the RLI in the E. coli. |
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