Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim
Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explo...
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| Format: | Thesis |
| Language: | en |
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2009
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| Online Access: | https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf https://ir.uitm.edu.my/id/eprint/105247/ |
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| _version_ | 1833322813217308672 |
|---|---|
| author | Mohamad Kassim, Muhamad Saifuzzaman |
| author_facet | Mohamad Kassim, Muhamad Saifuzzaman |
| author_sort | Mohamad Kassim, Muhamad Saifuzzaman |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explorer 1.2. Four out of six (All three 15kb PCR and one 20kb PCR) produce results. Two variables that affect the efficiencies of long range PCR were studied which consists of the percentage of DMSO added and the concentration of MgCb used in the PCR. A combination of 5%, 7%, and I 0% DMSO were added to study the improvement of the efficiencies of long range PCR amplification that theoretically should increase the ratio of full-length products to shortened products. Results were somehow unobtainable and optimum percentage of DMSO for long range PCR is not known. The differing MgCb concentrations were added and the result was also unobtainable and optimum concentration of free Mg2+ in the PCR mix is not known. |
| format | Thesis |
| id | my.uitm.ir-105247 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2009 |
| record_format | eprints |
| spelling | my.uitm.ir-1052472024-12-05T09:00:06Z https://ir.uitm.edu.my/id/eprint/105247/ Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim Mohamad Kassim, Muhamad Saifuzzaman Pharmaceutical industry Pharmaceutical chemistry Long range PCR method for uniform amplification of specific lambda DNA sequence is described. The careful design of all 6 pairs of primers and optimum temperature cycling profile produce good result for long range PCR. Three pairs of 15kb and three pairs of 20kb primers were design using oligo explorer 1.2. Four out of six (All three 15kb PCR and one 20kb PCR) produce results. Two variables that affect the efficiencies of long range PCR were studied which consists of the percentage of DMSO added and the concentration of MgCb used in the PCR. A combination of 5%, 7%, and I 0% DMSO were added to study the improvement of the efficiencies of long range PCR amplification that theoretically should increase the ratio of full-length products to shortened products. Results were somehow unobtainable and optimum percentage of DMSO for long range PCR is not known. The differing MgCb concentrations were added and the result was also unobtainable and optimum concentration of free Mg2+ in the PCR mix is not known. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim. (2009) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam). |
| spellingShingle | Pharmaceutical industry Pharmaceutical chemistry Mohamad Kassim, Muhamad Saifuzzaman Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim |
| title | Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim |
| title_full | Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim |
| title_fullStr | Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim |
| title_full_unstemmed | Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim |
| title_short | Investigation of the factors that influence long range PCR / Muhamad Saifuzzaman Mohamad Kassim |
| title_sort | investigation of the factors that influence long range pcr / muhamad saifuzzaman mohamad kassim |
| topic | Pharmaceutical industry Pharmaceutical chemistry |
| url | https://ir.uitm.edu.my/id/eprint/105247/1/105247.pdf https://ir.uitm.edu.my/id/eprint/105247/ |
| url_provider | http://ir.uitm.edu.my/ |