Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi
Determination of weak D types may reduce the risk of unnecessary alloimmunization and also beneficial for proper utilization of Rh negative blood units. Present study aimed to determine the weak D type among non-Chinese blood donors from National Blood Centre (NBC) Kuala Lumpur by using Polymerase c...
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| Format: | Thesis |
| Language: | en |
| Published: |
2016
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| Online Access: | https://ir.uitm.edu.my/id/eprint/101207/1/101207.pdf https://ir.uitm.edu.my/id/eprint/101207/ |
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| _version_ | 1833321961647767552 |
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| author | Hilmi, Mohd Sodiq Rifaei |
| author_facet | Hilmi, Mohd Sodiq Rifaei |
| author_sort | Hilmi, Mohd Sodiq Rifaei |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Determination of weak D types may reduce the risk of unnecessary alloimmunization and also beneficial for proper utilization of Rh negative blood units. Present study aimed to determine the weak D type among non-Chinese blood donors from National Blood Centre (NBC) Kuala Lumpur by using Polymerase chain reaction with specific sequence primer (PCR-SSP). Out of 104 Rh negative blood donors, only one turn out to be positive for weak D, but undefined to be detected by selected kits. The phenotype for the India sample were dCce. Rh phenotyping of all sample consist of 96% dce, 2.9% dCce, and 0.9% dCcEe. In conclusion, due to time constrained for sample collection and the use of kits, the weak D type undetermined. Hence, it is recommended to prolong the duration of the samples collection and to use the different weak D variant kits or using real time PCR. |
| format | Thesis |
| id | my.uitm.ir-101207 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2016 |
| record_format | eprints |
| spelling | my.uitm.ir-1012072024-08-27T17:12:42Z https://ir.uitm.edu.my/id/eprint/101207/ Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi Hilmi, Mohd Sodiq Rifaei Determination of weak D types may reduce the risk of unnecessary alloimmunization and also beneficial for proper utilization of Rh negative blood units. Present study aimed to determine the weak D type among non-Chinese blood donors from National Blood Centre (NBC) Kuala Lumpur by using Polymerase chain reaction with specific sequence primer (PCR-SSP). Out of 104 Rh negative blood donors, only one turn out to be positive for weak D, but undefined to be detected by selected kits. The phenotype for the India sample were dCce. Rh phenotyping of all sample consist of 96% dce, 2.9% dCce, and 0.9% dCcEe. In conclusion, due to time constrained for sample collection and the use of kits, the weak D type undetermined. Hence, it is recommended to prolong the duration of the samples collection and to use the different weak D variant kits or using real time PCR. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/101207/1/101207.pdf Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi. (2016) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/101207.pdf> |
| spellingShingle | Hilmi, Mohd Sodiq Rifaei Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi |
| title | Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi |
| title_full | Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi |
| title_fullStr | Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi |
| title_full_unstemmed | Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi |
| title_short | Molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (SSP-PCR) / Mohd Sodiq Rifaei Hilmi |
| title_sort | molecular analysis of weak d among non-chinese blood donors using specific sequence primer- polymerase chain reaction (ssp-pcr) / mohd sodiq rifaei hilmi |
| url | https://ir.uitm.edu.my/id/eprint/101207/1/101207.pdf https://ir.uitm.edu.my/id/eprint/101207/ |
| url_provider | http://ir.uitm.edu.my/ |