Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi
The study was done to identify the effect of culture condition on metabolite production by fungal strain 4PL2. fungi strain 4PL2 was isolated from plant of Pandanus herbaceous which was collected from Port Dickson, Negeri Sembilan in 2006. the study was performed by growing the pure culture of fungi...
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| Format: | Thesis |
| Language: | en |
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2008
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| Online Access: | https://ir.uitm.edu.my/id/eprint/101094/1/101094.PDF https://ir.uitm.edu.my/id/eprint/101094/ |
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| _version_ | 1833321929363161088 |
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| author | Mohd Abidi, Nor Azizah |
| author_facet | Mohd Abidi, Nor Azizah |
| author_sort | Mohd Abidi, Nor Azizah |
| building | Tun Abdul Razak Library |
| collection | Institutional Repository |
| content_provider | Universiti Teknologi Mara |
| content_source | UiTM Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | The study was done to identify the effect of culture condition on metabolite production by fungal strain 4PL2. fungi strain 4PL2 was isolated from plant of Pandanus herbaceous which was collected from Port Dickson, Negeri Sembilan in 2006. the study was performed by growing the pure culture of fungi strain 4PL2 in different media, different strength and different incubation period. The pure culture of 4PL2 was then incubated for 2 weeks, 3 weeks and 4 weeks. Then the myceliums of fungi strain 4PL2 were subjected to extraction process by using ethyl acetate as a solvent. After obtaining the pure extracts, then subjected to antimicrobial assay by using disc diffusion method. Three bacteria and two fungi have been used to assay the extract for antimicrobial activity and the result has been recorded. Consequently, the extract was analyzed using HPLC chromatogram to detect the production of major and minor metabolites for different media, different strength and different incubation period. All the data and information obtained from the observations, weight of extracts, microscopic pictures, antimicrobial test and HPLC chromatograms analysis were collected and recorded. |
| format | Thesis |
| id | my.uitm.ir-101094 |
| institution | Universiti Teknologi Mara |
| language | en |
| publishDate | 2008 |
| record_format | eprints |
| spelling | my.uitm.ir-1010942024-09-28T23:34:54Z https://ir.uitm.edu.my/id/eprint/101094/ Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi Mohd Abidi, Nor Azizah Pharmaceutical chemistry The study was done to identify the effect of culture condition on metabolite production by fungal strain 4PL2. fungi strain 4PL2 was isolated from plant of Pandanus herbaceous which was collected from Port Dickson, Negeri Sembilan in 2006. the study was performed by growing the pure culture of fungi strain 4PL2 in different media, different strength and different incubation period. The pure culture of 4PL2 was then incubated for 2 weeks, 3 weeks and 4 weeks. Then the myceliums of fungi strain 4PL2 were subjected to extraction process by using ethyl acetate as a solvent. After obtaining the pure extracts, then subjected to antimicrobial assay by using disc diffusion method. Three bacteria and two fungi have been used to assay the extract for antimicrobial activity and the result has been recorded. Consequently, the extract was analyzed using HPLC chromatogram to detect the production of major and minor metabolites for different media, different strength and different incubation period. All the data and information obtained from the observations, weight of extracts, microscopic pictures, antimicrobial test and HPLC chromatograms analysis were collected and recorded. 2008 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/101094/1/101094.PDF Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi. (2008) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam). |
| spellingShingle | Pharmaceutical chemistry Mohd Abidi, Nor Azizah Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi |
| title | Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi |
| title_full | Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi |
| title_fullStr | Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi |
| title_full_unstemmed | Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi |
| title_short | Effect of culture condition on metabolite using osmac approach produced by endophytic strain (4PL2) / Nor Azizah Mohd Abidi |
| title_sort | effect of culture condition on metabolite using osmac approach produced by endophytic strain (4pl2) / nor azizah mohd abidi |
| topic | Pharmaceutical chemistry |
| url | https://ir.uitm.edu.my/id/eprint/101094/1/101094.PDF https://ir.uitm.edu.my/id/eprint/101094/ |
| url_provider | http://ir.uitm.edu.my/ |