Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern
Insulin-like growth factor-1 (IGF-1) is a hormone comprising seventy amino acids that is a key regulator of muscle and bone health and is highly related to the development of cancer. An aptamer-antibody sandwich assay was performed to determine the presence of IGF1 on a gold nanoparticle-enhanced di...
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American Scientific Publishers
2022
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| Online Access: | http://eprints.sunway.edu.my/2964/ https://doi.org/10.1166/mex.2022.2153 |
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| author | Liu, Lanbo Gopinath, Subash C B Wu, Yuan Seng * Zhao, Weichao |
| author_facet | Liu, Lanbo Gopinath, Subash C B Wu, Yuan Seng * Zhao, Weichao |
| author_sort | Liu, Lanbo |
| building | Sunway Campus Library |
| collection | Institutional Repository |
| content_provider | Sunway University |
| content_source | Sunway Institutional Repository |
| continent | Asia |
| country | Malaysia |
| description | Insulin-like growth factor-1 (IGF-1) is a hormone comprising seventy amino acids that is a key regulator of muscle and bone health and is highly related to the development of cancer. An aptamer-antibody sandwich assay was performed to determine the presence of IGF1 on a gold nanoparticle-enhanced dielectrode junctional sensing surface by using this surface as a transducer. High-resolution microscopy observations showed the characteristics of the sensing surface and gold nanoparticles. The gold nanoparticle-enhanced surface induces high immobilization of the anti-IGF1 aptamer and enhances the interactions of IGF1. With this probe surface, the aptamer-IGF1-antibody sandwich assay resulted in an increased current for each different solution concentration of IGF1, and the detection limit was 0.5 pg/mL with an R 2 value of 0.9631 on a linear calibration curve with IGF1 solution concentrations of 1–16 pg/mL. In addition, IGF1-spiked serum reached a similar limit of detection of 0.5 pg/mL, confirming that selective IGF1 detection occurred for the biological sample. |
| format | Article |
| id | my.sunway.eprints.2964 |
| institution | Sunway University |
| publishDate | 2022 |
| publisher | American Scientific Publishers |
| record_format | eprints |
| spelling | my.sunway.eprints.29642024-08-04T02:01:52Z http://eprints.sunway.edu.my/2964/ Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern Liu, Lanbo Gopinath, Subash C B Wu, Yuan Seng * Zhao, Weichao QC Physics QH Natural history R Medicine (General) Insulin-like growth factor-1 (IGF-1) is a hormone comprising seventy amino acids that is a key regulator of muscle and bone health and is highly related to the development of cancer. An aptamer-antibody sandwich assay was performed to determine the presence of IGF1 on a gold nanoparticle-enhanced dielectrode junctional sensing surface by using this surface as a transducer. High-resolution microscopy observations showed the characteristics of the sensing surface and gold nanoparticles. The gold nanoparticle-enhanced surface induces high immobilization of the anti-IGF1 aptamer and enhances the interactions of IGF1. With this probe surface, the aptamer-IGF1-antibody sandwich assay resulted in an increased current for each different solution concentration of IGF1, and the detection limit was 0.5 pg/mL with an R 2 value of 0.9631 on a linear calibration curve with IGF1 solution concentrations of 1–16 pg/mL. In addition, IGF1-spiked serum reached a similar limit of detection of 0.5 pg/mL, confirming that selective IGF1 detection occurred for the biological sample. American Scientific Publishers 2022 Article PeerReviewed Liu, Lanbo and Gopinath, Subash C B and Wu, Yuan Seng * and Zhao, Weichao (2022) Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern. Materials Express, 12 (3). ISSN 2158-5849 https://doi.org/10.1166/mex.2022.2153 10.1166/mex.2022.2153 |
| spellingShingle | QC Physics QH Natural history R Medicine (General) Liu, Lanbo Gopinath, Subash C B Wu, Yuan Seng * Zhao, Weichao Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| title | Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| title_full | Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| title_fullStr | Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| title_full_unstemmed | Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| title_short | Gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| title_sort | gold-enhanced current-volt dielectrode junction for biosensing with an aptamer-insulin-like growth factor-1-antibody sandwich pattern |
| topic | QC Physics QH Natural history R Medicine (General) |
| url | http://eprints.sunway.edu.my/2964/ https://doi.org/10.1166/mex.2022.2153 |
| url_provider | http://eprints.sunway.edu.my/ |