Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient
Friedreich’s ataxia (FRDA) is a progressive neuromuscular disorder caused by substantial decrease of mitochondrial protein frataxin responsible for biogenesis of iron-sulphur clusters and protection from oxidative damage. In this study, we investigated the antioxidant activities of a standardized aq...
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2020
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| Online Access: | http://eprints.sunway.edu.my/1721/1/Yow%20Yoon%20Yen%20Antioxidant%20mediated.pdf http://eprints.sunway.edu.my/1721/ http://doi.org/10.1590/fst.09919 |
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| author | Lew, S. Y. Yow, Yoon Yen * Lim, L. W. Wong, K. H. |
| author_facet | Lew, S. Y. Yow, Yoon Yen * Lim, L. W. Wong, K. H. |
| author_sort | Lew, S. Y. |
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| content_provider | Sunway University |
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| continent | Asia |
| country | Malaysia |
| description | Friedreich’s ataxia (FRDA) is a progressive neuromuscular disorder caused by substantial decrease of mitochondrial protein frataxin responsible for biogenesis of iron-sulphur clusters and protection from oxidative damage. In this study, we investigated the antioxidant activities of a standardized aqueous extract from fruiting bodies of Hericium erinaceus mushroom (HESAE) and its protective effects against oxidative damage induced by L-Buthionine sulfoximine (BSO) in fibroblasts derived from FRDA patient. The lactate dehydrogenase-based viability assay showed that FRDA fibroblast was sensitive to 12.5 mM BSO with a reduction of viability to 52.51 ± 13.92% after 24 h of BSO exposure. Interestingly, co-incubation with 32 mg/mL HESAE increased the viability to 85.35 ± 3.4%. Further, 12.5 mM BSO caused a decrease in the ratio of cellular reduced glutathione (GSH) to oxidised GSH (GSSG) that leads to cell death. Nevertheless, the damage was reduced by co-incubation with 32 mg/mL HESAE. Nuclear fluorescence staining revealed that 12.5 mM BSO induced cell death and the apoptosis was decreased by co-incubation with HESAE. These findings suggest the ability of HESAE in attenuating BSO-mediated cytotoxicity through maintenance of membrane integrity and optimal GSH/GSSG ratio, that are closely linked to its antioxidant activities. Further in vivo trials are highly warranted to clarify its potential benefits in management of FRDA. |
| format | Article |
| id | my.sunway.eprints.1721 |
| institution | Sunway University |
| language | en |
| publishDate | 2020 |
| publisher | Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
| record_format | eprints |
| spelling | my.sunway.eprints.17212021-04-14T07:37:21Z http://eprints.sunway.edu.my/1721/ Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient Lew, S. Y. Yow, Yoon Yen * Lim, L. W. Wong, K. H. QH301 Biology Friedreich’s ataxia (FRDA) is a progressive neuromuscular disorder caused by substantial decrease of mitochondrial protein frataxin responsible for biogenesis of iron-sulphur clusters and protection from oxidative damage. In this study, we investigated the antioxidant activities of a standardized aqueous extract from fruiting bodies of Hericium erinaceus mushroom (HESAE) and its protective effects against oxidative damage induced by L-Buthionine sulfoximine (BSO) in fibroblasts derived from FRDA patient. The lactate dehydrogenase-based viability assay showed that FRDA fibroblast was sensitive to 12.5 mM BSO with a reduction of viability to 52.51 ± 13.92% after 24 h of BSO exposure. Interestingly, co-incubation with 32 mg/mL HESAE increased the viability to 85.35 ± 3.4%. Further, 12.5 mM BSO caused a decrease in the ratio of cellular reduced glutathione (GSH) to oxidised GSH (GSSG) that leads to cell death. Nevertheless, the damage was reduced by co-incubation with 32 mg/mL HESAE. Nuclear fluorescence staining revealed that 12.5 mM BSO induced cell death and the apoptosis was decreased by co-incubation with HESAE. These findings suggest the ability of HESAE in attenuating BSO-mediated cytotoxicity through maintenance of membrane integrity and optimal GSH/GSSG ratio, that are closely linked to its antioxidant activities. Further in vivo trials are highly warranted to clarify its potential benefits in management of FRDA. Sociedade Brasileira de Ciência e Tecnologia de Alimentos 2020 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1721/1/Yow%20Yoon%20Yen%20Antioxidant%20mediated.pdf Lew, S. Y. and Yow, Yoon Yen * and Lim, L. W. and Wong, K. H. (2020) Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient. Food Science and Technology, 40 (suppl). pp. 264-272. ISSN 0101-2061 http://doi.org/10.1590/fst.09919 doi:10.1590/fst.09919 |
| spellingShingle | QH301 Biology Lew, S. Y. Yow, Yoon Yen * Lim, L. W. Wong, K. H. Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient |
| title | Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient |
| title_full | Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient |
| title_fullStr | Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient |
| title_full_unstemmed | Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient |
| title_short | Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich’s ataxia patient |
| title_sort | antioxidant-mediated protective role of hericium erinaceus (bull.: fr.) pers. against oxidative damage in fibroblasts from friedreich’s ataxia patient |
| topic | QH301 Biology |
| url | http://eprints.sunway.edu.my/1721/1/Yow%20Yoon%20Yen%20Antioxidant%20mediated.pdf http://eprints.sunway.edu.my/1721/ http://doi.org/10.1590/fst.09919 |
| url_provider | http://eprints.sunway.edu.my/ |