Response surface optimization of rhizopus sp. immobilization onto loofah sponge using potato dextrose
The immobilization process has garnered significant attention in recent years, as it can help avoid the complex procedures of isolation and purification during the fermentation process. Loofah sponge (Luffa cylindrica) has gradually been used as an immobilizing matrix for various microorganisms d...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
King Mongkut's Institute of Technology Ladkrabang
2022
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/96685/1/96685_Response%20surface%20optimization%20of%20rhizopus.pdf http://irep.iium.edu.my/96685/ https://li01.tci-thaijo.org/index.php/cast/article/view/252685/173586 |
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| Summary: | The immobilization process has garnered significant attention
in recent years, as it can help avoid the complex procedures of isolation and purification during the fermentation process.
Loofah sponge (Luffa cylindrica) has gradually been used as
an immobilizing matrix for various microorganisms due to its
renewability and pore structure. In this study, the weight of
immobilized Rhizopus sp. onto loofah sponge was investigated using a face-centered central composite design
(FCCCD). Studied parameters included dextrose concentration (0.02-1.5 M), agitation speed (0-150 rpm), and incubation time (24-72 h). After two sets of experiments consisting of 17 and 11 runs, respectively, and based on the parameter proposed by FCCCD, a maximum biomass weight of 0.252 g was achieved onto 0.3 g of loofah at dextrose concentration of 0.45 M, agitation speed of 100 rpm, and incubation time of 24 h. The SEM results showed well-formed mycelia within and on the loofah surface with no sign of structural degradation. |
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