Step by step preparation of urine samples for in-Solution, gel-free proteomic studies, suitable for MALDI TOF MS and LCMS QTOF MS
Two-dimensional electrophoresis is an established method for isolating and separating proteins for proteomic analysis and identification of proteins. However, it requires a lot of optimization and it suffers from some ongoing concerns regarding quantitative reproducibility and limitations on its abi...
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| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | en |
| Published: |
Kulliyyah of Allied Health Sciences, International Islamic University Malaysia
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/90832/1/90832_Step%20by%20step%20preparation%20of%20urine.pdf http://irep.iium.edu.my/90832/ https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/622/504 |
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| Summary: | Two-dimensional electrophoresis is an established method for isolating and separating proteins for proteomic analysis and identification of proteins. However, it requires a lot of optimization and it suffers from some ongoing concerns regarding quantitative reproducibility and limitations on its ability to study certain class of proteins. We propose a simpler method in preparing urine samples from acute melioidosis patients for in-solution proteomics using liquid chromatography coupled with mass spectrometry quadrupole time of flight (LCMS-QTOF MS) or matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). This method is relatively easier than 2-dimensional electrophoresis, affordable, reproducible, can be performed in less equipped laboratories for transport to specialized centers for proteomic studies and bioinformatic analysis. |
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