Gas chromatography-mass spectrometry based metabolomics to Identify the alpha glucosidase inhibitors from flesh of salacca zalacca fruits and their molecular docking studies
Introduction: Salak fruit (Salacca zalacca) is traditionally used and commercialized as an antidiabetic agent. However, the scientific evidence to proof this usage is quiet lacking. Objectives: This research was aimed to evaluate the antidiabetic activity of S. zalacca fruit using α-glucosidase...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Medknow
2020
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/85875/1/JPBS_Abstract_ID%20101.pdf http://irep.iium.edu.my/85875/ http://www.jpbsonline.org |
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| Summary: | Introduction: Salak fruit (Salacca zalacca) is traditionally
used and commercialized as an antidiabetic agent. However, the scientific evidence to proof this usage is quiet lacking.
Objectives: This research was aimed to evaluate the
antidiabetic activity of S. zalacca fruit using α-glucosidase
enzyme and profiling the identified antidiabetic agents
using gas chromatography-mass spectrometry (GCMS).
Material and Methods: For metabolomics study,
the fruit was extracted by different ratios of ethanol
and water (0, 20, 40, 60, 80, 100%, v/v) to get E0 (100%
water), E20 (20% ethanol), E40 (40% ethanol), E60 (60%
ethanol), E80 (80% ethanol) and E100 (100% ethanol).
The α-glucosidase enzyme was used in determining
the α-glucosidase inhibitory activity. Results: The 60%
aqueous ethanolic extract exhibited the highest bioactivity
(IC50 16.90 μg/mL). Several α-glucosidase inhibitors such
as stearic acid, palmitic acid, β-Sitosterol, myo-inositol
were identified by GC-MS. The docking results predicted
the interactions of all the metabolites identified to be in
non-competitive mode involving majorly hydrophobic
interactions with the protein residues. Some of the residues
involved are PRO456, AGR467, LYS406 HID295, ASN259
LEU313, LYS125 H-bond HIE315, ARG315, PHE303.
Conclusion: The in vitro assay strongly suggests that E60
extract from S. zalacca fruit is having potential antidiabetic
activity. Addition of extra hydrophobic groups on palmitic
acid and ß-sitosterol would increase the inhibitory activity. |
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