Site-directed mutagenesis on plasmid using polymerase chain reaction

Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid ha...

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Bibliographic Details

Main Authors:	Ahmad, Abdul Aziz, Mohd. Salleh, Hamzah, Noorbatcha, Ibrahim Ali
Format:	Book Chapter
Language:	en en
Published:	Springer Nature 2018
Subjects:	TP200 Manufacture and use of chemicals TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf http://irep.iium.edu.my/70243/ https://www.springer.com/us/book/9789811322563 https://doi.org/10.1007/978-981-13-2257-0
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