Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1- picrylhydrazyl ( DPPH) radical...
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UPENA (UiTM Press)
2018
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| Online Access: | http://irep.iium.edu.my/67921/1/journalgading2018.pdf http://irep.iium.edu.my/67921/ https://gadingst.learningdistance.org/index.php/gadingst/article/view/10/6 |
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| author | Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian |
| author_facet | Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian |
| author_sort | Harun, Aiza |
| building | IIUM Library |
| collection | Institutional Repository |
| content_provider | International Islamic University Malaysia |
| content_source | IIUM Repository (IREP) |
| continent | Asia |
| country | Malaysia |
| description | In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali
from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant
properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-
picrylhydrazyl ( DPPH) radical scavenging activity. The isolation of antioxidative
compounds was conducted through preparative thin layer chromatography with the use of
certain binary solvents. The structure determination was carried out using Gas
Chromatography Mass Spectrometer (GCMS). The methanol extracts from both stem and
leaf exhibited highest antioxidant activity with lowest IC50 of 1.14 ug/ml and 81 ug/ml
respectively compared to other extracts. The stem showed stronger antioxidant activity
compared to leaves part. Based on GCMS analysis, the antioxidative compounds isolated
from leaves part were proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7atetrahydrobenzofuran-2(4H)-one
(1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene1,1,4,4-tetrone
(2), Methyl stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and
9-Oxononanoic acid (6). The antioxidative compounds isolated from stem part were
determined as oleic acid (7), 1-(hydroxymethyl)-1,2- ethanediyl ester (8) and 9-octadecene
(9). This finding can be used as scientific baseline information for the future treatment of
diseases caused by harmful free radicals. |
| format | Article |
| id | my.iium.irep-67921 |
| institution | Universiti Islam Antarabangsa Malaysia |
| language | en |
| publishDate | 2018 |
| publisher | UPENA (UiTM Press) |
| record_format | dspace |
| spelling | my.iium.irep-679212018-12-05T01:43:15Z http://irep.iium.edu.my/67921/ Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian QD Chemistry In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1- picrylhydrazyl ( DPPH) radical scavenging activity. The isolation of antioxidative compounds was conducted through preparative thin layer chromatography with the use of certain binary solvents. The structure determination was carried out using Gas Chromatography Mass Spectrometer (GCMS). The methanol extracts from both stem and leaf exhibited highest antioxidant activity with lowest IC50 of 1.14 ug/ml and 81 ug/ml respectively compared to other extracts. The stem showed stronger antioxidant activity compared to leaves part. Based on GCMS analysis, the antioxidative compounds isolated from leaves part were proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7atetrahydrobenzofuran-2(4H)-one (1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene1,1,4,4-tetrone (2), Methyl stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and 9-Oxononanoic acid (6). The antioxidative compounds isolated from stem part were determined as oleic acid (7), 1-(hydroxymethyl)-1,2- ethanediyl ester (8) and 9-octadecene (9). This finding can be used as scientific baseline information for the future treatment of diseases caused by harmful free radicals. UPENA (UiTM Press) 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67921/1/journalgading2018.pdf Harun, Aiza and Daud, Shaari and Harith, Siti Suhaila and Ab Rahim, Asmira and Abd Jalil, Mohd Azfar and Muhammad Rosdi, Nurul ‘Atiqah and , Fatin Azieyati and , Naila Nabihah and Mat So'ad, Siti Zaiton and Mohd Hassan, Norazian (2018) Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species. Gading Journal for Science and Technology, 1 (1). pp. 52-59. E-ISSN 2637-0018 https://gadingst.learningdistance.org/index.php/gadingst/article/view/10/6 |
| spellingShingle | QD Chemistry Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_full | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_fullStr | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_full_unstemmed | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_short | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_sort | radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of leea indica species |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/67921/1/journalgading2018.pdf http://irep.iium.edu.my/67921/ https://gadingst.learningdistance.org/index.php/gadingst/article/view/10/6 |
| url_provider | http://irep.iium.edu.my/ |