Effect of Tualang honey on the regulation of liver lipid metabolizing enzymes gene expression in a 12% high cholesterol diet-induced obese rats
Abstract Obesity disrupts liver lipid metabolism by altering lipid-metabolizing enzyme expression. Although treatments for obesity exist, their use is often limited by adverse effects, leading to increasing interest in natural products for treating obesity. Thus, this study aims to investigate th...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en |
| Published: |
Universiti Putra Malaysia
2025
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/122008/7/122008_Effect%20of%20Tualang%20honey.pdf http://irep.iium.edu.my/122008/ https://www.msmbb.my/index.php/publication |
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| Summary: | Abstract
Obesity disrupts liver lipid metabolism by altering lipid-metabolizing enzyme expression. Although
treatments for obesity exist, their use is often limited by adverse effects, leading to increasing
interest in natural products for treating obesity. Thus, this study aims to investigate the effect of
Tualang honey (TH) on liver lipid-metabolizing enzyme gene expression in obese rats due to their
beneficial biological properties. Thirty male Sprague-Dawley rats were randomly assigned into five
groups (n=6 per group). All rats were initially fed their respective diets for 12 weeks, followed by
specific treatments for the subsequent 6 weeks. Group 1 was fed a normal diet (ND) without
treatment, while Group 2 received a normal diet supplemented with TH at 3.0 g/kg. Group 3 was
given a 12% high-cholesterol diet (HCD) alone, whereas Group 4 received a 12% HCD treated
with TH (3.0 g/kg). Lastly, Group 5 was provided with a 12% HCD treated with Orlistat (10
mg/kg). At the end, the rats were sacrificed, livers excised for lipid-metabolizing enzymes gene
expression study. Gene expression of acetyl-CoA carboxylase (ACC), sterol regulatory element
binding protein-1 (SREBP-1), and HMG-CoA reductase was significantly upregulated, while
carnitine palmitoyl transferase-1 (CPT-1) was significantly downregulated in Group 3 compared
to Group 1 (p<0.01). When compared to Group 3, TH supplementation in Group 4 significantly
downregulated ACC (1.7-fold), SREBP-1 (1.3-fold), and HMG-CoA reductase (1.5-fold) while
upregulating CPT-1 expression (0.7-fold) (p<0.01). TH may exert its anti-obesity effects by
regulating the expression of genes involved in lipid metabolism. |
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