Evaluation of pollen viability by colorimetric analysis of guava cultivars
Pollen viability is important for the success of sexual reproduction in plants. Based on viability, pollen quality is assessed in order to improve fruit quality and productivity. The study focus at evaluating the pollen viability of three different guava cultivars in Malaysia. The colorimetric met...
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| Main Authors: | , |
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| Format: | Proceeding Paper |
| Language: | en en |
| Published: |
2022
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/100948/1/2ndASRIC%202022%20Athirah-Huda.pdf http://irep.iium.edu.my/100948/7/PROGRAMME%20BOOK%20ASRIC2022.pdf http://irep.iium.edu.my/100948/ |
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| Summary: | Pollen viability is important for the success of sexual reproduction in plants. Based on viability, pollen quality is
assessed in order to improve fruit quality and productivity. The study focus at evaluating the pollen viability of
three different guava cultivars in Malaysia. The colorimetric method of estimation of pollen viability was done
by using 2,3,5-triphenyl tetrazolium chloride (TTC) and Iodine Potassium Iodide (IKI) solution. The observation
for the viability of pollen were made at x40 magnification using a light microscope. IKI staining has higher pollen
viability percentage (97.93 - 99.59%) in all guava species compared to TTC (2.29% - 59.26%). The results of the
Mann-Whitney test showed that the differences in pollen viability under IKI and TTC staining were significant at
p < 0.05, z = -6.685. The highest viable pollen were recorded from Lohan, followed by Pink guava heirloom and
the least from Kim Choo red. However, Kruskal-Wallis analysis at p < 0.05 revealed that the distribution of pollen
viability among cultivars was not significantly different. According to these observations, viability obtained by
staining methods are easier and faster, but may frequently yields erroneous positive findings. Thus, other test such
as in vitro germination method may be useful to study the occurrence of pollen viability. |
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