Antimicrobial activity of β-Sitosterol Isolated from Kalanchoe tomentosa leaves against Staphylococcus aureus and Klebsiella pneumonia
Background and Objective: Fresh K. tomentosa leaves obtained from Bandung, Indonesia was extracted using n-hexane followed by serial dichloromethane maceration. Material and Method: N-hexane and ethyl acetate were used to separate the dichloromethane extract using Vacuum Liquid Chromatography and th...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en en |
| Published: |
Asian Network for Scientific Information
2022
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/100938/7/100938_Antimicrobial%20activity%20of%20%CE%B2-Sitosterol%20Isolated.pdf http://irep.iium.edu.my/100938/13/100938_Antimicrobial%20activity%20of%20%CE%B2-Sitosterol%20Isolated_Scopus.pdf http://irep.iium.edu.my/100938/ https://scialert.net/abstract/?doi=pjbs.2022.602.607 |
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| Summary: | Background and Objective: Fresh K. tomentosa leaves obtained from Bandung, Indonesia was extracted using n-hexane followed by serial dichloromethane maceration. Material and Method: N-hexane and ethyl acetate were used to separate the dichloromethane extract using Vacuum Liquid Chromatography and the isolated compounds were recrystallized with n-hexane. Result: 37 mg of dichloromethane extract was obtained from the extraction process. Recrystallized compound isolates were identified as stigmast-5-en-3ꞵ-ol or β-sitosterol. Both dichloromethane extract and β-sitosterol isolated compounds showed strong bacteriostatic activity against S. aureus with MIC=15.63 µg/mL and 7.81µg/mL, and K. pneumonia with MIC=7.81µg/mL and 31.25µg/mL, respectively. However, only dichloromethane extract exhibited a bactericidal effect (7.81 µg/mL). Conclusion: The pure β-sitosterol compound was isolated from K. tomentosa dichloromethane extract. Both the dichloromethane extract and the isolated β-sitosterol compound had antibacterial effects against S. aureus and K. pneumonia |
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