Screening and identification of potential green polylactic acid (GPLA)-degrading bacteria isolated from biofertilizer samples
Plastic pollution has emerged as a global issue that caused negative impact to the environment and human health. This has led to the development of biodegradable plastic, such as polylactic acid (PLA) to reduce the plastic hazards. Green polylactic acid (GPLA) is originated from the PLA family wh...
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| Format: | Final Year Project / Dissertation / Thesis |
| Published: |
2024
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| Subjects: | |
| Online Access: | http://eprints.utar.edu.my/6474/1/2001565_Dissertation.pdf http://eprints.utar.edu.my/6474/ |
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| Summary: | Plastic pollution has emerged as a global issue that caused negative impact to the
environment and human health. This has led to the development of
biodegradable plastic, such as polylactic acid (PLA) to reduce the plastic hazards.
Green polylactic acid (GPLA) is originated from the PLA family which is
derived from renewable sources with an enhanced biodegradability. Biofertilizer
is frequently used in the agricultural sector to improve plant growth and crop
yields. It contains microbial agents which possess potential in plastic
biodegradation. In this project, bacterial isolates from biofertilizer samples were
screened for GPLA degradation using clear zone assay and identified by 16S
rDNA sequencing. The bacterial isolates were incubated on M9 screening media
supplemented with different composition of GPLA powder at 30°C for a week.
Coomassie Brilliant Blue solution was employed to visualise the formation of
clear zone. Bacterial isolates that demonstrated zone of clearance were selected
to perform genomic DNA extraction using phenol chloroform method, followed by PCR amplification of the 16S rDNA encoding gene and sequence analysis.
The sequencing results were compared to the database of NCBI using BLAST
programme to determine the identity of the potential GPLA degraders. The
results showed that two bacterial isolates were able to produce clear zone on 0.2,
0.4, and 0.6% GPLA screening plates. Based on the 16S rDNA sequence analysis,
the bacterial isolates were identified as Brucella intermedia and
Stenotrophomonas maltophilia strain W1-2. Further evaluation of GPLA
degradation by B. intermedia and S. maltophilia is required to understand the
biodegradation mechanisms. This study provided insights in the application of
microorganisms in the biofertilizer for plastic waste management. |
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