Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes
Southeast Asia generates over 8 million tons of vegetable waste annually, yet the microbial isolations from these wastes are rarely associated with potent probiotics. This study aimed to screen lactic acid bacteria (LAB) from fermented and non-fermented vegetable wastes for probiotic potential. LAB...
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/26548/1/SMD%204.pdf http://journalarticle.ukm.my/26548/ https://www.ukm.my/jsm/english_journals/vol54num12_2025/contentsVol54num12_2025.html |
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| author | Nur Hidayahanum Hamid, Mohd Fakhrulddin Ismail, Hassan Mohd Daud, Srisapoome, Prapansak Murni Marlina Abd Karim, Ahmad Zaharin Aris, Ruhil Hayati Hamdan, Siti Fairus Mohamed Yusof, |
| author_facet | Nur Hidayahanum Hamid, Mohd Fakhrulddin Ismail, Hassan Mohd Daud, Srisapoome, Prapansak Murni Marlina Abd Karim, Ahmad Zaharin Aris, Ruhil Hayati Hamdan, Siti Fairus Mohamed Yusof, |
| author_sort | Nur Hidayahanum Hamid, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Southeast Asia generates over 8 million tons of vegetable waste annually, yet the microbial isolations from these wastes are rarely associated with potent probiotics. This study aimed to screen lactic acid bacteria (LAB) from fermented and non-fermented vegetable wastes for probiotic potential. LAB were isolated using serial dilutions on MRS agar supplemented with 1% CaCO3 , yielding 17 isolates. Two isolates, FCU21 and FBS34, demonstrated notable probiotic potential by exhibiting antibacterial activity against Aeromonas hydrophila (6.3 ± 0.08 mm and 6.2 ± 0.04 mm) and Streptococcus agalactiae (5.6 ± 0.04 mm and 10.4 ± 0.28 mm). Initial identification based on morphological, phenotypic and biochemical characteristics showed Gram-positive, catalase-negative, non-spore-forming cocci. Genotypic analysis using 16S rRNA sequencing confirmed both isolates as Enterococcus hirae, with 99% similarity for FCU21 (from fermented cucumber) and 100% for FBS34 (from fermented mung bean sprouts), designated as Enterococcus hirae UPM01 and UPM02, respectively. Whole-genome shotgun sequencing showed distinct genomic features, including variations in base pairs, contigs and coding sequences. These findings highlight the promising potential of Enterococcus hirae strains as probiotics for aquaculture, warranting further investigation into their probiotic properties and applications. |
| format | Article |
| id | my-ukm.journal.26548 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.265482026-02-11T01:30:41Z http://journalarticle.ukm.my/26548/ Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes Nur Hidayahanum Hamid, Mohd Fakhrulddin Ismail, Hassan Mohd Daud, Srisapoome, Prapansak Murni Marlina Abd Karim, Ahmad Zaharin Aris, Ruhil Hayati Hamdan, Siti Fairus Mohamed Yusof, Southeast Asia generates over 8 million tons of vegetable waste annually, yet the microbial isolations from these wastes are rarely associated with potent probiotics. This study aimed to screen lactic acid bacteria (LAB) from fermented and non-fermented vegetable wastes for probiotic potential. LAB were isolated using serial dilutions on MRS agar supplemented with 1% CaCO3 , yielding 17 isolates. Two isolates, FCU21 and FBS34, demonstrated notable probiotic potential by exhibiting antibacterial activity against Aeromonas hydrophila (6.3 ± 0.08 mm and 6.2 ± 0.04 mm) and Streptococcus agalactiae (5.6 ± 0.04 mm and 10.4 ± 0.28 mm). Initial identification based on morphological, phenotypic and biochemical characteristics showed Gram-positive, catalase-negative, non-spore-forming cocci. Genotypic analysis using 16S rRNA sequencing confirmed both isolates as Enterococcus hirae, with 99% similarity for FCU21 (from fermented cucumber) and 100% for FBS34 (from fermented mung bean sprouts), designated as Enterococcus hirae UPM01 and UPM02, respectively. Whole-genome shotgun sequencing showed distinct genomic features, including variations in base pairs, contigs and coding sequences. These findings highlight the promising potential of Enterococcus hirae strains as probiotics for aquaculture, warranting further investigation into their probiotic properties and applications. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/26548/1/SMD%204.pdf Nur Hidayahanum Hamid, and Mohd Fakhrulddin Ismail, and Hassan Mohd Daud, and Srisapoome, Prapansak and Murni Marlina Abd Karim, and Ahmad Zaharin Aris, and Ruhil Hayati Hamdan, and Siti Fairus Mohamed Yusof, (2025) Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes. Sains Malaysiana, 54 (12). pp. 2847-2858. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num12_2025/contentsVol54num12_2025.html |
| spellingShingle | Nur Hidayahanum Hamid, Mohd Fakhrulddin Ismail, Hassan Mohd Daud, Srisapoome, Prapansak Murni Marlina Abd Karim, Ahmad Zaharin Aris, Ruhil Hayati Hamdan, Siti Fairus Mohamed Yusof, Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| title | Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| title_full | Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| title_fullStr | Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| title_full_unstemmed | Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| title_short | Screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| title_sort | screening and preliminary characterization of potential probiotic lactic acid bacteria from vegetable wastes |
| url | http://journalarticle.ukm.my/26548/1/SMD%204.pdf http://journalarticle.ukm.my/26548/ https://www.ukm.my/jsm/english_journals/vol54num12_2025/contentsVol54num12_2025.html |
| url_provider | http://journalarticle.ukm.my/ |