Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia
Acute myeloid leukaemia (AML) is a severe bone marrow malignancy with a high mortality rate. Recent advancements in high throughput technology and bioinformatics have facilitated the identification of microRNAs (miRNAs) involved in the pathogenesis of AML. This study aimed to investigate the potenti...
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| Language: | en |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/26540/1/ML%2014.pdf http://journalarticle.ukm.my/26540/ https://jms.mabjournal.com/index.php/mab/issue/view/69 |
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| author | Ezalia Esa, Wan Muhammad Farhan Syafiq Wan Mohd Nor, Elsa Haniffah Mejia Mohamed, Lim, Wei-Yue Zubaidah Zakaria, Yuslina Mat Yusoff, Pau, Ivyna Ni Bong Pung, Yuh-Fen Noor Atiqah Fakharuzi, Shamsul Mohd Zain, |
| author_facet | Ezalia Esa, Wan Muhammad Farhan Syafiq Wan Mohd Nor, Elsa Haniffah Mejia Mohamed, Lim, Wei-Yue Zubaidah Zakaria, Yuslina Mat Yusoff, Pau, Ivyna Ni Bong Pung, Yuh-Fen Noor Atiqah Fakharuzi, Shamsul Mohd Zain, |
| author_sort | Ezalia Esa, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Acute myeloid leukaemia (AML) is a severe bone marrow malignancy with a high mortality rate. Recent advancements in high throughput technology and bioinformatics have facilitated the identification of microRNAs (miRNAs) involved in the pathogenesis of AML. This study aimed to investigate the potential role of MicroRNA-let-7i-5p (miR-let-7i-5p) in the progression of AML. MiR let-7 family has been widely utilised as a tumour inhibitor, suppressing its expression in several types of human cancer. Hence, dysregulation of miR-let-7i-5p is critical to cancer progression. However, the role of miR-let-7i-5p in AML remains unclear. Using the Gene Expression Omnibus (GEO) database, the expression of miR-let-7i-5p in AML patients was investigated. The role of miR-let-7i-5p and its potential target genes in AML was examined using bioinformatic tools. Subsequently, the effect of miR-let-7i 5p was assessed through transfection, followed by the analysis of target gene expression using RT-qPCR. Further investigations employed the MTT and cell cycle assays on the effects of miR-let-7i-5p on AML cell proliferation and cell cycle. According to the GEO database, miR-let-7i-5p was significantly downregulated in AML patients compared to controls. AML development has been associated with miR-let-7i-5p by KEGG pathway analysis. Bioinformatics suggested that miR-let-7i-5p targets MYC in AML cells. Transfection of miR-let-7i-5p into AML cell lines decreased MYC expression, suggesting MYC regulates AML progression. However, MTT and cell cycle assays showed no significant effect of miR-let-7i-5p on AML cell proliferation and cell cycle, indicating the potential involvement of miR-let-7i-5p in alternative pathways in AML pathogenesis. The study highlights the potential significance of miR-let-7i-5p in regulating AML progression. |
| format | Article |
| id | my-ukm.journal.26540 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.265402026-02-06T01:26:59Z http://journalarticle.ukm.my/26540/ Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia Ezalia Esa, Wan Muhammad Farhan Syafiq Wan Mohd Nor, Elsa Haniffah Mejia Mohamed, Lim, Wei-Yue Zubaidah Zakaria, Yuslina Mat Yusoff, Pau, Ivyna Ni Bong Pung, Yuh-Fen Noor Atiqah Fakharuzi, Shamsul Mohd Zain, Acute myeloid leukaemia (AML) is a severe bone marrow malignancy with a high mortality rate. Recent advancements in high throughput technology and bioinformatics have facilitated the identification of microRNAs (miRNAs) involved in the pathogenesis of AML. This study aimed to investigate the potential role of MicroRNA-let-7i-5p (miR-let-7i-5p) in the progression of AML. MiR let-7 family has been widely utilised as a tumour inhibitor, suppressing its expression in several types of human cancer. Hence, dysregulation of miR-let-7i-5p is critical to cancer progression. However, the role of miR-let-7i-5p in AML remains unclear. Using the Gene Expression Omnibus (GEO) database, the expression of miR-let-7i-5p in AML patients was investigated. The role of miR-let-7i-5p and its potential target genes in AML was examined using bioinformatic tools. Subsequently, the effect of miR-let-7i 5p was assessed through transfection, followed by the analysis of target gene expression using RT-qPCR. Further investigations employed the MTT and cell cycle assays on the effects of miR-let-7i-5p on AML cell proliferation and cell cycle. According to the GEO database, miR-let-7i-5p was significantly downregulated in AML patients compared to controls. AML development has been associated with miR-let-7i-5p by KEGG pathway analysis. Bioinformatics suggested that miR-let-7i-5p targets MYC in AML cells. Transfection of miR-let-7i-5p into AML cell lines decreased MYC expression, suggesting MYC regulates AML progression. However, MTT and cell cycle assays showed no significant effect of miR-let-7i-5p on AML cell proliferation and cell cycle, indicating the potential involvement of miR-let-7i-5p in alternative pathways in AML pathogenesis. The study highlights the potential significance of miR-let-7i-5p in regulating AML progression. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/26540/1/ML%2014.pdf Ezalia Esa, and Wan Muhammad Farhan Syafiq Wan Mohd Nor, and Elsa Haniffah Mejia Mohamed, and Lim, Wei-Yue and Zubaidah Zakaria, and Yuslina Mat Yusoff, and Pau, Ivyna Ni Bong and Pung, Yuh-Fen and Noor Atiqah Fakharuzi, and Shamsul Mohd Zain, (2025) Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia. Malaysian Applied Biology, 54 (4). pp. 146-153. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/69 |
| spellingShingle | Ezalia Esa, Wan Muhammad Farhan Syafiq Wan Mohd Nor, Elsa Haniffah Mejia Mohamed, Lim, Wei-Yue Zubaidah Zakaria, Yuslina Mat Yusoff, Pau, Ivyna Ni Bong Pung, Yuh-Fen Noor Atiqah Fakharuzi, Shamsul Mohd Zain, Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia |
| title | Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia |
| title_full | Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia |
| title_fullStr | Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia |
| title_full_unstemmed | Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia |
| title_short | Mir-let-7i-5p as a novel regulator of MYC gene expression in acute myeloid leukaemia |
| title_sort | mir-let-7i-5p as a novel regulator of myc gene expression in acute myeloid leukaemia |
| url | http://journalarticle.ukm.my/26540/1/ML%2014.pdf http://journalarticle.ukm.my/26540/ https://jms.mabjournal.com/index.php/mab/issue/view/69 |
| url_provider | http://journalarticle.ukm.my/ |