Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production
Global demand for lactic acid (LA) has increased significantly in recent years, thus, prompting increased interest in utilising lignocellulosic waste biomass for its production. This study investigated the relationship between cellulase activity and LA production by lactic acid bacteria (LAB) isolat...
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/26513/1/SSS%206.pdf http://journalarticle.ukm.my/26513/ https://www.ukm.my/jsm/english_journals/vol54num11_2025/contentsVol54num11_2025.html |
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| author | Sideeqot Toyin Abdullahi, Ahmad Faris Mohd Adnan, Mohammad Moneruzzaman Khandaker, Wakisaka, Minato |
| author_facet | Sideeqot Toyin Abdullahi, Ahmad Faris Mohd Adnan, Mohammad Moneruzzaman Khandaker, Wakisaka, Minato |
| author_sort | Sideeqot Toyin Abdullahi, |
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| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Global demand for lactic acid (LA) has increased significantly in recent years, thus, prompting increased interest in utilising lignocellulosic waste biomass for its production. This study investigated the relationship between cellulase activity and LA production by lactic acid bacteria (LAB) isolated from the gut of rhinoceros beetle (Oryctes rhinoceros) larvae. Out of 11 LAB strains tested, three isolates namely L5-5, L2a-1 and L3-2 were observed to exhibit good growth using glucose as substrate and were able to obtain final LA concentrations of 9.04 g/L, 9.26 g/L and 9.34 g/L, respectively. These strains were further screened for cellulolytic activity at various temperatures and pH levels using a carboxymethyl cellulose (CMC) assay and were identified as Enterococcus thailandicus through 16S rDNA sequencing. Optimal enzyme activity at 45 °C for strain L5-5 was 0.42 U/mL, L2a-1 was 0.61 U/mL, and L3-2 was 0.62 U/mL. Notably, the strong cellulolytic activity was positively correlated with the elevated LA production. These LAB strains could tolerate a broad temperature range of 30 to 50 °C, salt concentrations of 2.5 to 10 % (w/v), furfural concentration of up to 1% (v/v), and pH levels between 4.5 and 9.0. The findings highlighted the potential of LAB isolates from O. rhinoceros larval gut as viable candidates for LA production in industrial applications. |
| format | Article |
| id | my-ukm.journal.26513 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.265132026-02-09T06:47:42Z http://journalarticle.ukm.my/26513/ Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production Sideeqot Toyin Abdullahi, Ahmad Faris Mohd Adnan, Mohammad Moneruzzaman Khandaker, Wakisaka, Minato Global demand for lactic acid (LA) has increased significantly in recent years, thus, prompting increased interest in utilising lignocellulosic waste biomass for its production. This study investigated the relationship between cellulase activity and LA production by lactic acid bacteria (LAB) isolated from the gut of rhinoceros beetle (Oryctes rhinoceros) larvae. Out of 11 LAB strains tested, three isolates namely L5-5, L2a-1 and L3-2 were observed to exhibit good growth using glucose as substrate and were able to obtain final LA concentrations of 9.04 g/L, 9.26 g/L and 9.34 g/L, respectively. These strains were further screened for cellulolytic activity at various temperatures and pH levels using a carboxymethyl cellulose (CMC) assay and were identified as Enterococcus thailandicus through 16S rDNA sequencing. Optimal enzyme activity at 45 °C for strain L5-5 was 0.42 U/mL, L2a-1 was 0.61 U/mL, and L3-2 was 0.62 U/mL. Notably, the strong cellulolytic activity was positively correlated with the elevated LA production. These LAB strains could tolerate a broad temperature range of 30 to 50 °C, salt concentrations of 2.5 to 10 % (w/v), furfural concentration of up to 1% (v/v), and pH levels between 4.5 and 9.0. The findings highlighted the potential of LAB isolates from O. rhinoceros larval gut as viable candidates for LA production in industrial applications. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/26513/1/SSS%206.pdf Sideeqot Toyin Abdullahi, and Ahmad Faris Mohd Adnan, and Mohammad Moneruzzaman Khandaker, and Wakisaka, Minato (2025) Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production. Sains Malaysiana, 54 (11). pp. 2645-2659. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num11_2025/contentsVol54num11_2025.html |
| spellingShingle | Sideeqot Toyin Abdullahi, Ahmad Faris Mohd Adnan, Mohammad Moneruzzaman Khandaker, Wakisaka, Minato Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| title | Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| title_full | Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| title_fullStr | Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| title_full_unstemmed | Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| title_short | Homofermentative lactic acid-and cellulase-secreting Oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| title_sort | homofermentative lactic acid-and cellulase-secreting oryctes rhinoceros larval gut bacterial strains for lactic acid production |
| url | http://journalarticle.ukm.my/26513/1/SSS%206.pdf http://journalarticle.ukm.my/26513/ https://www.ukm.my/jsm/english_journals/vol54num11_2025/contentsVol54num11_2025.html |
| url_provider | http://journalarticle.ukm.my/ |