Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris
Cordyceps militaris is a valuable medicinal mushroom due to its bioactive components, especially cordycepin and adenosine, which have significant pharmacological activities. However, the yield and quality of fruiting bodies and their active compounds can vary greatly depending on the nutritional con...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | en |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2025
|
| Online Access: | http://journalarticle.ukm.my/26404/1/MT%2014.pdf http://journalarticle.ukm.my/26404/ https://jms.mabjournal.com/index.php/mab/issue/view/68 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1854094004665188352 |
|---|---|
| author | Thao, Hoang Xuan Hao, Nguyen Thị Hoi, Truong Cong Hai, Le Thanh Trang, Le Thi Thuy Nhi, Nguyen Thi Ngoc Minh, Le Hoang Duy Giang, Tran Van |
| author_facet | Thao, Hoang Xuan Hao, Nguyen Thị Hoi, Truong Cong Hai, Le Thanh Trang, Le Thi Thuy Nhi, Nguyen Thi Ngoc Minh, Le Hoang Duy Giang, Tran Van |
| author_sort | Thao, Hoang Xuan |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Cordyceps militaris is a valuable medicinal mushroom due to its bioactive components, especially cordycepin and adenosine, which have significant pharmacological activities. However, the yield and quality of fruiting bodies and their active compounds can vary greatly depending on the nutritional conditions during cultivation. This study aimed to determine the optimal culture medium and extraction procedure to enhance both biomass yield and bioactive compound content in C. militaris. The mushroom was cultivated under three nutritional conditions: Basal control medium, T1 medium supplemented with soybean powder and coconut water, and T2 medium supplemented with mineral salts (MgSO4 , K2 SO4 ) without soybean powder or coconut water. Growth performance, fruiting body morphology, and cordycepin/adenosine content were evaluated, followed by optimization of extraction efficiency using Box–Behnken design, taking into account extraction temperature, ethanol/mushroom ratio, and extraction time. T1 provided the most favorable conditions for the development of C. militaris fruiting bodies compared to the control group, including the indices stem length (9.82 ± 1.44 cm), diameter (0.83 ± 0.16 cm), number of fruiting bodies (185.88 ± 19.53), and weight (17.18 ± 2.73 g). HPLC analysis showed the highest content of cordycepin (337.93 ± 15.08 mg/100 g) and adenosine (394.51 ± 10.86 mg/100 g). Therefore, we used group T1 to evaluate the effects of temperature, ethanol/fungi ratio, and extraction time on extraction efficiency (17 treatments). The results showed that treatment 17, with the conditions of 60°C, ethanol/fungi ratio 20:1, 6 hr, gave the best extraction efficiency of 33.15%. These results showed that the addition of nutrients from soybean and coconut water and the factors (temperature, ethanol/fungi ratio, and extraction time) significantly affected both the biomass and bioactive compound content of C. militaris as well as the extraction efficiency, providing a basis for improving the large-scale cultivation and extraction process for functional food and pharmaceutical applications. |
| format | Article |
| id | my-ukm.journal.26404 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.264042026-01-11T08:37:04Z http://journalarticle.ukm.my/26404/ Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris Thao, Hoang Xuan Hao, Nguyen Thị Hoi, Truong Cong Hai, Le Thanh Trang, Le Thi Thuy Nhi, Nguyen Thi Ngoc Minh, Le Hoang Duy Giang, Tran Van Cordyceps militaris is a valuable medicinal mushroom due to its bioactive components, especially cordycepin and adenosine, which have significant pharmacological activities. However, the yield and quality of fruiting bodies and their active compounds can vary greatly depending on the nutritional conditions during cultivation. This study aimed to determine the optimal culture medium and extraction procedure to enhance both biomass yield and bioactive compound content in C. militaris. The mushroom was cultivated under three nutritional conditions: Basal control medium, T1 medium supplemented with soybean powder and coconut water, and T2 medium supplemented with mineral salts (MgSO4 , K2 SO4 ) without soybean powder or coconut water. Growth performance, fruiting body morphology, and cordycepin/adenosine content were evaluated, followed by optimization of extraction efficiency using Box–Behnken design, taking into account extraction temperature, ethanol/mushroom ratio, and extraction time. T1 provided the most favorable conditions for the development of C. militaris fruiting bodies compared to the control group, including the indices stem length (9.82 ± 1.44 cm), diameter (0.83 ± 0.16 cm), number of fruiting bodies (185.88 ± 19.53), and weight (17.18 ± 2.73 g). HPLC analysis showed the highest content of cordycepin (337.93 ± 15.08 mg/100 g) and adenosine (394.51 ± 10.86 mg/100 g). Therefore, we used group T1 to evaluate the effects of temperature, ethanol/fungi ratio, and extraction time on extraction efficiency (17 treatments). The results showed that treatment 17, with the conditions of 60°C, ethanol/fungi ratio 20:1, 6 hr, gave the best extraction efficiency of 33.15%. These results showed that the addition of nutrients from soybean and coconut water and the factors (temperature, ethanol/fungi ratio, and extraction time) significantly affected both the biomass and bioactive compound content of C. militaris as well as the extraction efficiency, providing a basis for improving the large-scale cultivation and extraction process for functional food and pharmaceutical applications. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/26404/1/MT%2014.pdf Thao, Hoang Xuan and Hao, Nguyen Thị and Hoi, Truong Cong and Hai, Le Thanh and Trang, Le Thi Thuy and Nhi, Nguyen Thi Ngoc and Minh, Le Hoang Duy and Giang, Tran Van (2025) Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris. Malaysian Applied Biology, 54 (3). pp. 149-159. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/68 |
| spellingShingle | Thao, Hoang Xuan Hao, Nguyen Thị Hoi, Truong Cong Hai, Le Thanh Trang, Le Thi Thuy Nhi, Nguyen Thi Ngoc Minh, Le Hoang Duy Giang, Tran Van Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris |
| title | Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris |
| title_full | Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris |
| title_fullStr | Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris |
| title_full_unstemmed | Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris |
| title_short | Evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of Cordyceps militaris |
| title_sort | evaluating the effects of different nutritional conditions on fruiting body development and extraction efficiency of cordyceps militaris |
| url | http://journalarticle.ukm.my/26404/1/MT%2014.pdf http://journalarticle.ukm.my/26404/ https://jms.mabjournal.com/index.php/mab/issue/view/68 |
| url_provider | http://journalarticle.ukm.my/ |