Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.)
HMG-CoA reductase is an enzyme that converts HMG-CoA into cholesterol via the mevalonate pathway, contributing to cardiovascular disease. The germination of brown kara kratok (Phaseolus lunatus) mixtures, including dark brown, light brown, or brown color pattern, specifically for 0, 24, 48, 72, and...
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25633/1/SE%2010.pdf http://journalarticle.ukm.my/25633/ https://www.ukm.my/jsm/english_journals/vol54num4_2025/contentsVol54num4_2025.html |
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| author | Budiyanto, Cahyo Ningrum, Andriati Murdiati, Agnes Indrati, Retno |
| author_facet | Budiyanto, Cahyo Ningrum, Andriati Murdiati, Agnes Indrati, Retno |
| author_sort | Budiyanto, Cahyo |
| building | Tun Sri Lanang Library |
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| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | HMG-CoA reductase is an enzyme that converts HMG-CoA into cholesterol via the mevalonate pathway, contributing to cardiovascular disease. The germination of brown kara kratok (Phaseolus lunatus) mixtures, including dark brown, light brown, or brown color pattern, specifically for 0, 24, 48, 72, and 96 h, yielded interesting results. The samples were measured for radicle length, % inhibition of HMG-CoA reductase, type of inhibition, IC50 value, total protein content, amino acids, percentage degree of hydrolysis (%DH), and molecular weight (MW), providing valuable insights into the potential use of these biopeptides. The results showed that biopeptides from germinated kara kratok exhibited the ability as HMG-CoA reductase inhibitors. Furthermore, the germination process increased its capability, from 46.79% in beans to 83.77% in 72-h germination. The 72-h germination was observed to be the best HMG-CoA reductase inhibitor as a competitive inhibitor, with an IC50 value of 335.09 μg/mL. This was supported by a total protein content of 27.76%, with glutamic acid (2.99%) as the dominant amino acid, followed by phenylalanine, aspartic acid, leucine, serine, and arginine, %DH (26.26%), and MW (5-15 kDa). Brown kara kratok sprouts possess the potential to inhibit HMG-CoA reductase, and germination increases its capability. |
| format | Article |
| id | my-ukm.journal.25633 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.256332025-07-21T07:39:05Z http://journalarticle.ukm.my/25633/ Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) Budiyanto, Cahyo Ningrum, Andriati Murdiati, Agnes Indrati, Retno HMG-CoA reductase is an enzyme that converts HMG-CoA into cholesterol via the mevalonate pathway, contributing to cardiovascular disease. The germination of brown kara kratok (Phaseolus lunatus) mixtures, including dark brown, light brown, or brown color pattern, specifically for 0, 24, 48, 72, and 96 h, yielded interesting results. The samples were measured for radicle length, % inhibition of HMG-CoA reductase, type of inhibition, IC50 value, total protein content, amino acids, percentage degree of hydrolysis (%DH), and molecular weight (MW), providing valuable insights into the potential use of these biopeptides. The results showed that biopeptides from germinated kara kratok exhibited the ability as HMG-CoA reductase inhibitors. Furthermore, the germination process increased its capability, from 46.79% in beans to 83.77% in 72-h germination. The 72-h germination was observed to be the best HMG-CoA reductase inhibitor as a competitive inhibitor, with an IC50 value of 335.09 μg/mL. This was supported by a total protein content of 27.76%, with glutamic acid (2.99%) as the dominant amino acid, followed by phenylalanine, aspartic acid, leucine, serine, and arginine, %DH (26.26%), and MW (5-15 kDa). Brown kara kratok sprouts possess the potential to inhibit HMG-CoA reductase, and germination increases its capability. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25633/1/SE%2010.pdf Budiyanto, Cahyo and Ningrum, Andriati and Murdiati, Agnes and Indrati, Retno (2025) Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.). Sains Malaysiana, 54 (4). pp. 1089-1099. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num4_2025/contentsVol54num4_2025.html |
| spellingShingle | Budiyanto, Cahyo Ningrum, Andriati Murdiati, Agnes Indrati, Retno Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) |
| title | Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) |
| title_full | Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) |
| title_fullStr | Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) |
| title_full_unstemmed | Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) |
| title_short | Enhanced functional characteristics as a cholesterol-lowering bioactive peptide from Kara Kratok sprouts (Phaseolus lunatus L.) |
| title_sort | enhanced functional characteristics as a cholesterol-lowering bioactive peptide from kara kratok sprouts (phaseolus lunatus l.) |
| url | http://journalarticle.ukm.my/25633/1/SE%2010.pdf http://journalarticle.ukm.my/25633/ https://www.ukm.my/jsm/english_journals/vol54num4_2025/contentsVol54num4_2025.html |
| url_provider | http://journalarticle.ukm.my/ |