Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration
Osteoarthritis is characterized by the progressive deterioration of articular cartilage, leading to joint pain and functional impairments. Current treatment options are limited in their ability to stimulate cartilage regeneration. Evidence suggests that the co-culture technique, involving the interp...
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| Format: | Article |
| Language: | en |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24973/1/SD%2018.pdf http://journalarticle.ukm.my/24973/ https://www.ukm.my/jsm/english_journals/vol53num12_2024/contentsVol53num12_2024.html |
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| _version_ | 1834435078993739776 |
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| author | Muhammad Faizan Tariq, Sumera Rashid, Aisha Tarar, Umar Sajjad, Muhammad Rauf Ahmed, Bushra Ijaz, Haiba Kaul, Noreen Latief, |
| author_facet | Muhammad Faizan Tariq, Sumera Rashid, Aisha Tarar, Umar Sajjad, Muhammad Rauf Ahmed, Bushra Ijaz, Haiba Kaul, Noreen Latief, |
| author_sort | Muhammad Faizan Tariq, |
| building | Tun Sri Lanang Library |
| collection | Institutional Repository |
| content_provider | Universiti Kebangsaan Malaysia |
| content_source | UKM Journal Article Repository |
| continent | Asia |
| country | Malaysia |
| description | Osteoarthritis is characterized by the progressive deterioration of articular cartilage, leading to joint pain and functional impairments. Current treatment options are limited in their ability to stimulate cartilage regeneration. Evidence suggests that the co-culture technique, involving the interplay of multiple cell types, may effectively restore damaged cartilage. This investigation assessed the regenerative impact of co-culture on osteoarthritis-affected rat knee joints. Gene expression profiling validated phenotypic and biochemical expression analysis. Compared to the control group, the co-cultivated cohorts showed elevated levels of cartilage-specific markers, such as collagen and aggrecan. Notably, the group with stress and co-cultured with normal and osteoarthritic chondrocytes (H2O2+N+OA) demonstrated significant results, including lower LDH release (15.06 ± 1.461), decreased glycosaminoglycan levels (1.551 ± 0.1487), and reduced cell death percentage (17.50 ± 3.536) compared to the H2O2 control. Safranin-O staining retention also increased (28.89 ± 2.846), indicating enhanced cartilage matrix retention. Enhanced expression of survival markers such as Bcl (0.3974 ± 0.02241) and decreased apoptotic markers like Bax (0.2961 ± 0.01199) were observed, confirming the stimulation of critical genes involved in cartilage development and matrix synthesis. These findings support the potential of co-culture technology to accelerate cartilage regeneration and offer an innovative strategy to impede osteoarthritis progression. |
| format | Article |
| id | my-ukm.journal.24973 |
| institution | Universiti Kebangsaan Malaysia |
| language | en |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| record_format | eprints |
| spelling | my-ukm.journal.249732025-03-19T07:14:29Z http://journalarticle.ukm.my/24973/ Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration Muhammad Faizan Tariq, Sumera Rashid, Aisha Tarar, Umar Sajjad, Muhammad Rauf Ahmed, Bushra Ijaz, Haiba Kaul, Noreen Latief, Osteoarthritis is characterized by the progressive deterioration of articular cartilage, leading to joint pain and functional impairments. Current treatment options are limited in their ability to stimulate cartilage regeneration. Evidence suggests that the co-culture technique, involving the interplay of multiple cell types, may effectively restore damaged cartilage. This investigation assessed the regenerative impact of co-culture on osteoarthritis-affected rat knee joints. Gene expression profiling validated phenotypic and biochemical expression analysis. Compared to the control group, the co-cultivated cohorts showed elevated levels of cartilage-specific markers, such as collagen and aggrecan. Notably, the group with stress and co-cultured with normal and osteoarthritic chondrocytes (H2O2+N+OA) demonstrated significant results, including lower LDH release (15.06 ± 1.461), decreased glycosaminoglycan levels (1.551 ± 0.1487), and reduced cell death percentage (17.50 ± 3.536) compared to the H2O2 control. Safranin-O staining retention also increased (28.89 ± 2.846), indicating enhanced cartilage matrix retention. Enhanced expression of survival markers such as Bcl (0.3974 ± 0.02241) and decreased apoptotic markers like Bax (0.2961 ± 0.01199) were observed, confirming the stimulation of critical genes involved in cartilage development and matrix synthesis. These findings support the potential of co-culture technology to accelerate cartilage regeneration and offer an innovative strategy to impede osteoarthritis progression. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24973/1/SD%2018.pdf Muhammad Faizan Tariq, and Sumera Rashid, and Aisha Tarar, and Umar Sajjad, and Muhammad Rauf Ahmed, and Bushra Ijaz, and Haiba Kaul, and Noreen Latief, (2024) Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration. Sains Malaysiana, 53 (12). pp. 3349-3364. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num12_2024/contentsVol53num12_2024.html |
| spellingShingle | Muhammad Faizan Tariq, Sumera Rashid, Aisha Tarar, Umar Sajjad, Muhammad Rauf Ahmed, Bushra Ijaz, Haiba Kaul, Noreen Latief, Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| title | Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| title_full | Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| title_fullStr | Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| title_full_unstemmed | Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| title_short | Cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| title_sort | cytoprotective role of chondrocyte coculturing for enhanced cartilage regeneration |
| url | http://journalarticle.ukm.my/24973/1/SD%2018.pdf http://journalarticle.ukm.my/24973/ https://www.ukm.my/jsm/english_journals/vol53num12_2024/contentsVol53num12_2024.html |
| url_provider | http://journalarticle.ukm.my/ |